PNPLA1 blocking peptide

PNPLA1 Antibody (N-term) Blocking peptide

• Gene Names: PNPLA1; ARCI10; dJ50J22.1
• Synonyms: PNPLA1; PNPLA1 Antibody (N-term) Blocking peptide; Patatin-like phospholipase domain-containing protein 1; 311-; PNPLA1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of PNPLA1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 532

• Cellular Location: Cytoplasm. Note: Clearly distributed in the cytoplasm of keratinocytes
• Tissue Location: Expressed in the digestive system. Expressed in the epidermis of skin keratinocytes. Strongly expressed in the granular layer. Expressed in the upper epidermis and eccrine sweat glands of the dermis and in the region of keratin filament bundles, which is more pronounced in upper epidermal layers and in the lower cornified layers.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PNPLA1 blocking peptide

Lipid hydrolase. Important in the formation of the epidermal lipid barrier. Plays a role in glycerophospholipid metabolism.

NCBI and Uniprot Product Information

• NCBI GI #: 296452995
• NCBI GeneID: 285848
• UniProt Accession #: Q8N8W4; Q2A6N1; Q3SY95; Q3SY96; Q5R3L2; A3RMU3; J3JS20
• Molecular Weight: 48,884 Da
• NCBI Official Full Name: Patatin-like phospholipase domain-containing protein 1
• NCBI Official Synonym Full Names: patatin like phospholipase domain containing 1
• NCBI Official Symbol: PNPLA1
• NCBI Official Synonym Symbols: ARCI10; dJ50J22.1
• NCBI Protein Information: patatin-like phospholipase domain-containing protein 1
• UniProt Protein Name: Patatin-like phospholipase domain-containing protein 1
• Protein Family: Patatin-like phospholipase domain-containing protein
• UniProt Gene Name: PNPLA1
• UniProt Entry Name: PLPL1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the patatin-like phospholipase (PNPLA) family, which is characterized by the presence of a highly conserved patatin domain. PNPLA family members have diverse lipolytic and acyltransferase activities, and are key elements in lipid metabolism. While other members of this family have been well characterized, the function of this gene remained an enigma. However, recent studies show that this gene is expressed in the skin epidermal keratinocytes, and has a role in glycerophospholipid metabolism in the cutaneous barrier. Consistent with these observations, mutations in this gene are associated with ichthyosis in human (autosomal recessive congenital ichthyoses, ARCI) and dog. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

PNPLA1: Lipid hydrolase. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.1.-; Hydrolase

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: cytoplasm

Disease: Ichthyosis, Congenital, Autosomal Recessive 10