PHF1 blocking peptide

PHF1 Blocking Peptide

Cat. Num. AAA9623201

• Gene Names: PHF1; PCL1; PHF2; hPHF1; MTF2L2; TDRD19C
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: PHF1; PHF1 Blocking Peptide; hPCl1; MTF2 L2; MTF2L 2; MTF2L2; PCL 1; PCL1; PHD finger protein 1; PHD finger protein1; PHF 1; PHF 2; Phf1; PHF1_HUMAN; PHF2; Plc 1; Plc1; Polycomb like protein 1; Polycomb like protein1; Polycomb-like protein 1; Protein PHF1; T complex testis expressed 3; T complex testis expressed3; Tctex 3; Tctex3; PHF1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for PHF1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PHF1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4505777
• NCBI GeneID: 5252
• NCBI Accession #: NP_002627.1
• NCBI GenBank Nucleotide #: NM_002636.4
• UniProt Accession #: O43189; O60929; Q5SU07; Q5SU08; Q96KM7; B1AZX2; B1AZX3
• Molecular Weight: 62,106 Da
• NCBI Official Full Name: PHD finger protein 1 isoform a
• NCBI Official Synonym Full Names: PHD finger protein 1
• NCBI Official Symbol: PHF1
• NCBI Official Synonym Symbols: PCL1; PHF2; hPHF1; MTF2L2; TDRD19C
• NCBI Protein Information: PHD finger protein 1; hPCl1; polycomb-like protein 1; tudor domain containing 19C
• UniProt Protein Name: PHD finger protein 1
• Protein Family: PHD finger protein
• UniProt Gene Name: PHF1
• UniProt Synonym Gene Names: PCL1; Protein PHF1; hPHF1; hPCl1
• UniProt Entry Name: PHF1_HUMAN

NCBI Description

This gene encodes a Polycomb group protein. The protein is a component of a histone H3 lysine-27 (H3K27)-specific methyltransferase complex, and functions in transcriptional repression of homeotic genes. The protein is also recruited to double-strand breaks, and reduced protein levels results in X-ray sensitivity and increased homologous recombination. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

PHF1: Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: nucleoplasm; ESC/E(Z) complex; cytoplasm; microtubule organizing center; nucleus

Molecular Function: zinc ion binding; transcription factor activity; methylated histone residue binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of gene expression, epigenetic; gene expression; chromatin modification; response to DNA damage stimulus; regulation of gene expression, epigenetic