Form/Format
Antigenic Blocking Peptide
Concentration
Quantity: 250 ug; Volume: 100 ul (varies by lot)
Applicable Applications for PDE4A8 blocking peptide
Immunodepletion, Immunocompetition
Application Notes
Confocal Microscopy||1:200
Immunocytochemistry: 1:200
Immunofluorescence: 1:200
Immunohistochemistry: 1:200
Western Blot: 1:1,000
Immunogen
Synthetic Peptide
Expression
Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Iso
Molecular Function
metal ion binding, 3', 5'-cyclic-AMP phosphodiesterase activity, cAMP binding
Structure
Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2.
Subcellular Location
Isoform 1: Cytoplasm, perinuclear region; Isoform 2: Cytoplasm, perinuclear region. Cell projection, ruffle membrane; Isoform 4: Peripheral membrane protein; Isoform 6: Cytoplasm; perinuclear region; Isoform 7: Cytoplasm, Membrane
Preparation and Storage
-20 degree C for long term storage
Related Product Information for PDE4A8 blocking peptide
Antigenic Blocking Peptide cAMP-specific 3', 5'-cyclic phosphodiesterase 4A Antibody
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
NCBI and Uniprot Product Information
Molecular Weight
95,571 Da
NCBI Official Full Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4A
NCBI Official Synonym Full Names
phosphodiesterase 4A
NCBI Official Symbol
PDE4A
NCBI Official Synonym Symbols
PDE4; DPDE2; PDE46
NCBI Protein Information
cAMP-specific 3',5'-cyclic phosphodiesterase 4A
UniProt Protein Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4A
UniProt Synonym Gene Names
DPDE2
UniProt Entry Name
PDE4A_HUMAN
NCBI Description
The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Uniprot Description
PDE4A: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. Isoform 1 interacts with LYN. Isoform 2 and isoform 6 interact weakly with LYN. Isoform 1, isoform 2 and isoform 6 interact with ARRB2. Isoform 1 is widely expressed. Isoform 2 is abundant in liver, stomach, testis, thyroid and adrenal glands. It is also found in placenta, kidney, pancreas, ovary, uterus, skin, monocytes, mast cells, macrophages, as well as in bronchial smooth muscle. Isoform 6 is expressed at high levels in the heart and small intestine. It is also found in the brain, kidney, spleen, colon, salivary gland, ovary and peripheral blood lymphocytes. Isoform 7 is expressed predominantly in skeletal muscle and brain and at lower levels in the testis. Isoform 7 is expressed in the brain. Found in specific neuronal subpopulations in cortex, spinal cord and cerebellum. Isoform 1, isoform 2, isoform 6 and isoform 7 are inhibited by rolipram and cilomilast. Isoform 1, isoform 2 and isoform 6 are inhibited by 4-[(3-butoxy-4-methoxyphenyl)-methyl]- 2-imidazolidinone (Ro 20-1724), roflumilast and denbufylline. Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Phosphodiesterase; Nucleotide Metabolism - purine; EC 3.1.4.53
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: cytosol; membrane; perinuclear region of cytoplasm
Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; cAMP binding; metal ion binding; protein binding
Biological Process: cAMP catabolic process; sensory perception of smell; signal transduction
Research Articles on PDE4A8
Similar Products
Product Notes
The PDE4A8 pde4a (Catalog #
AAA544196) is a Blocking Peptide and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PDE4A8 can be used in a range of immunoassay formats including, but not limited to, Immunodepletion, Immunocompetition. Confocal Microscopy||1:200 Immunocytochemistry: 1:200 Immunofluorescence: 1:200 Immunohistochemistry: 1:200 Western Blot: 1:1,000. Researchers should empirically determine the suitability of the PDE4A8 pde4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
It is sometimes possible for the material contained within the vial of
"PDE4A8, Blocking Peptide" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are
absolutely not suitable for use in any
sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a
product from AAA Biotech, you
are explicitly certifying that said products will be properly tested and used in line with
industry standard. AAA Biotech
and its authorized distribution partners reserve the right to refuse to fulfill any order if
we have any indication that a
purchaser may be intending to use a product outside of our accepted criteria.
Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech
cannot be held
responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any
aspect of this datasheet at
any time and without notice. It is the responsibility of the customer to inform AAA Biotech
of any product performance
issues observed or experienced within 30 days of receipt of said product. To see additional
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