PAK4 blocking peptide

PAK4 Antibody (N-term) Blocking Peptide

• Synonyms: PAK4; PAK4 Antibody (N-term) Blocking Peptide; Serine/threonine-protein kinase PAK 4; p21-activated kinase 4; PAK-4; KIAA1142; PAK4 blocking peptide

• Clone Number: 2040109
• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human PAK4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 591

• Cellular Location: Cytoplasm. Note: Seems to shuttle between cytoplasmic compartments depending on the activating effector. For example, can be found on the cell periphery after activation of growth-factor or integrin-mediated signaling pathways
• Tissue Location: Highest expression in prostate, testis and colon
• Function: Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Related Product Information for PAK4 blocking peptide

PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3, PAK4, PAK5, and PAK6. PAK proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton .

NCBI and Uniprot Product Information

• NCBI GI #: 12585288
• NCBI GeneID: 10298
• UniProt Accession #: O96013; Q8N4E1; Q8NCH5; Q8NDE3; Q9BU33; Q9ULS8; B4DGG6
• NCBI Official Full Name: Serine/threonine-protein kinase PAK 4
• NCBI Official Synonym Full Names: p21 (RAC1) activated kinase 4
• NCBI Official Symbol: PAK4
• NCBI Protein Information: serine/threonine-protein kinase PAK 4
• UniProt Protein Name: Serine/threonine-protein kinase PAK 4
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK4
• UniProt Synonym Gene Names: KIAA1142; PAK-4
• UniProt Entry Name: PAK4_HUMAN

NCBI Description

PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PAK4: a protein kinase of the STE20 family. A mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Interacts specifically with the GTP-bound form of Cdc42 and weakly activates the JNK family of MAP kinases. Overexpressed in several cancers. Required for ras-dependent anchorage-independent growth of tumor cell lines. Four alternatively spliced human isoforms have been reported.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; PAKB subfamily

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: focal adhesion; Golgi apparatus

Molecular Function: protein binding; receptor signaling protein serine/threonine kinase activity

Biological Process: apoptosis; cell growth; cell migration; cell motility; cell proliferation; cytoskeleton organization and biogenesis; mitotic cell cycle; signal transduction