OGG1 blocking peptide

OGG1 Blocking Peptide

Cat. Num. AAA9625774

• Gene Names: OGG1; HMMH; MUTM; OGH1; HOGG1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: OGG1; OGG1 Blocking Peptide; 8 hydroxyguanine DNA glycosylase; 8 oxoguanine DNA glycosylase 1; 8 oxoguanine DNA glycosylase; AP lyase; DNA (apurinic or apyrimidinic site) lyase; DNA apurinic or apyrimidinic site lyase; DNA lyase; DNA-(apurinic or apyrimidinic site) lyase; HMMH; HOGG 1; HOGG1; MMH; MUTM; N glycosylase; Ogg 1; Ogg1; OGG1_HUMAN; OGH 1; OGH1; OGG1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for OGG1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for OGG1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4505495
• NCBI GeneID: 4968
• NCBI Accession #: NP_002533.1
• NCBI GenBank Nucleotide #: NM_002542.5
• UniProt Accession #: O15527; O00390; O00670; O00705; O14876; O95488; P78554; Q9BW42; Q9UIK0; Q9UIK1; Q9UIK2; A8K1E3
• Molecular Weight: 36,085 Da
• NCBI Official Full Name: N-glycosylase/DNA lyase isoform 1a
• NCBI Official Synonym Full Names: 8-oxoguanine DNA glycosylase
• NCBI Official Symbol: OGG1
• NCBI Official Synonym Symbols: HMMH; MUTM; OGH1; HOGG1
• NCBI Protein Information: N-glycosylase/DNA lyase; 8-hydroxyguanine DNA glycosylase; AP lyase; DNA-apurinic or apyrimidinic site lyase; OGG1 type 1f
• UniProt Protein Name: N-glycosylase/DNA lyase
• Protein Family: N-glycosylase/DNA lyase
• UniProt Gene Name: OGG1
• UniProt Synonym Gene Names: MMH; MUTM; OGH1; AP lyase
• UniProt Entry Name: OGG1_HUMAN

NCBI Description

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]

Uniprot Description

OGG1: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N- methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta- lyase activity that nicks DNA 3' to the lesion. Defects in OGG1 may be a cause of renal cell carcinoma (RCC). It is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into clear cell renal carcinoma (non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma. Belongs to the type-1 OGG1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Lyase; Deoxyribonuclease; EC 4.2.99.18; DNA repair, damage

Chromosomal Location of Human Ortholog: 3p26.2

Cellular Component: nucleoplasm; nuclear matrix; mitochondrion; nuclear speck

Molecular Function: protein binding; endonuclease activity; microtubule binding; oxidized purine base lesion DNA N-glycosylase activity; DNA N-glycosylase activity; damaged DNA binding; 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity

Biological Process: response to drug; depurination; DNA repair; DNA catabolic process, endonucleolytic; response to estradiol stimulus; response to radiation; response to ethanol; base-excision repair, AP site formation; regulation of transcription, DNA-dependent; base-excision repair; nucleotide-excision repair; response to folic acid; regulation of protein import into nucleus, translocation; response to oxidative stress; acute inflammatory response; negative regulation of apoptosis; aging

Disease: Renal Cell Carcinoma, Nonpapillary