NSF blocking peptide

NSF Blocking Peptide (C-term)

• Gene Names: NSF; SKD2
• Synonyms: NSF; NSF Blocking Peptide (C-term); Vesicle-fusing ATPase; N-ethylmaleimide-sensitive fusion protein; NEM-sensitive fusion protein; Vesicular-fusion protein NSF; NSF blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 683-696 of HUMAN NSF
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 744

• Cellular Location: Cytoplasm.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NSF blocking peptide

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 156564401
• NCBI GeneID: 4905
• NCBI Accession #: NP_006169.2
• NCBI GenBank Nucleotide #: NM_006178.3
• UniProt Accession #: P46459; Q8N6D7; Q9UKZ2; A8K2D9; B4DFA2
• Molecular Weight: 71,584 Da
• NCBI Official Full Name: vesicle-fusing ATPase
• NCBI Official Synonym Full Names: N-ethylmaleimide sensitive factor, vesicle fusing ATPase
• NCBI Official Symbol: NSF
• NCBI Official Synonym Symbols: SKD2
• NCBI Protein Information: vesicle-fusing ATPase
• UniProt Protein Name: Vesicle-fusing ATPase
• Protein Family: NSFL1 cofactor
• UniProt Gene Name: NSF
• UniProt Synonym Gene Names: NEM-sensitive fusion protein
• UniProt Entry Name: NSF_HUMAN

Uniprot Description

NSF: required for intracellular membrane fusion and vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Functions as a SNAP receptor (SNARE) chaperone which binds, through soluble NSF attachment proteins (SNAPs), to SNARE complexes and utilizes the energy of ATP hydrolysis to disassemble them thus facilitating SNARE recycling. While this is a major function of NSF, it does seem to interact with other proteins, such as the AMPA receptor subunit, GluR2, and beta2-AR and is thought to affect their trafficking patterns.

Protein type: Autophagy; Vesicle; Adaptor/scaffold; EC 3.6.4.6; Protease

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cytoplasm; cytosol; dendritic shaft; Golgi stack; lysosomal membrane; plasma membrane; postsynaptic density

Molecular Function: ATPase activity; PDZ domain binding; protein binding; protein complex binding; protein kinase binding; Rab GTPase binding; syntaxin-1 binding

Biological Process: COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; exocytosis; Golgi to plasma membrane protein transport; Golgi vesicle docking; intra-Golgi vesicle-mediated transport; intracellular protein transport; plasma membrane fusion; positive regulation of protein catabolic process; positive regulation of receptor recycling; regulation of exocytosis; retrograde vesicle-mediated transport, Golgi to ER; vesicle-mediated transport