NOS3 blocking peptide

NOS3 Antibody (N-term) Blocking Peptide

• Gene Names: NOS3; eNOS; ECNOS
• Synonyms: NOS3; NOS3 Antibody (N-term) Blocking Peptide; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII; NOS3 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human NOS3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1203

• Cellular Location: Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity
• Tissue Location: Platelets, placenta, liver and kidney.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NOS3 blocking peptide

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

NCBI and Uniprot Product Information

• NCBI GI #: 266648
• NCBI GeneID: 4846
• UniProt Accession #: P29474; Q13662; Q14251; Q14434; Q548C1; Q6GSL5; Q9UDC6; A0S0A7; A0S0A8; A8KA63; B2RCQ1; E9PFR2
• Molecular Weight: 67,876 Da
• NCBI Official Full Name: Nitric oxide synthase, endothelial
• NCBI Official Synonym Full Names: nitric oxide synthase 3
• NCBI Official Symbol: NOS3
• NCBI Official Synonym Symbols: eNOS; ECNOS
• NCBI Protein Information: nitric oxide synthase, endothelial
• UniProt Protein Name: Nitric oxide synthase, endothelial
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS3
• UniProt Synonym Gene Names: cNOS; eNOS; NOSIII
• UniProt Entry Name: NOS3_HUMAN

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.

Protein type: EC 1.14.13.39; Oxidoreductase; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: caveola; cytoplasm; cytosol; Golgi membrane; nucleus; plasma membrane

Molecular Function: actin monomer binding; heme binding; nitric-oxide synthase activity; protein binding

Biological Process: arginine catabolic process; blood vessel remodeling; cell redox homeostasis; endothelial cell migration; mitochondrion organization and biogenesis; negative regulation of blood pressure; negative regulation of cell proliferation; negative regulation of muscle hyperplasia; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; positive regulation of angiogenesis; positive regulation of guanylate cyclase activity; positive regulation of vasodilation; regulation of blood vessel size; regulation of nitric-oxide synthase activity; regulation of systemic arterial blood pressure by endothelin; removal of superoxide radicals; smooth muscle hyperplasia

Disease: Alzheimer Disease; Hypertension, Essential; Preeclampsia/eclampsia 1; Stroke, Ischemic