NCSTN blocking peptide

NCSTN Blocking Peptide (N-Term)

• Gene Names: NCSTN; ATAG1874
• Synonyms: NCSTN; NCSTN Blocking Peptide (N-Term); Nicastrin; KIAA0253; NCSTN blocking peptide

• Specificity: The synthetic peptide sequence is selected from aa 99-113 of HUMAN NCSTN
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 709

• Cellular Location: Membrane; Single-pass type I membrane protein. Melanosome Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV
• Tissue Location: Widely expressed.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for NCSTN blocking peptide

Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.

NCBI and Uniprot Product Information

• NCBI GI #: 12231037
• NCBI GeneID: 23385
• UniProt Accession #: Q92542; Q5T207; Q5T208; Q86VV5
• Molecular Weight: 76,744 Da
• NCBI Official Full Name: Nicastrin
• NCBI Official Synonym Full Names: nicastrin
• NCBI Official Symbol: NCSTN
• NCBI Official Synonym Symbols: ATAG1874
• NCBI Protein Information: nicastrin
• UniProt Protein Name: Nicastrin
• Protein Family: Nicastrin
• UniProt Gene Name: NCSTN
• UniProt Synonym Gene Names: KIAA0253
• UniProt Entry Name: NICA_HUMAN

NCBI Description

This gene encodes a type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Mutations in this gene are associated with familial acne inversa. A pseudogene of this gene is present on chromosome 21. Alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Feb 2014]

Uniprot Description

nicastrin: Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP). Constitutively expressed in neural cells. Widely expressed. Belongs to the nicastrin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22-q23

Cellular Component: endoplasmic reticulum; focal adhesion; Golgi apparatus; integral to plasma membrane; lysosomal membrane; membrane; plasma membrane

Molecular Function: protein binding

Biological Process: amyloid precursor protein catabolic process; ephrin receptor signaling pathway; membrane protein ectodomain proteolysis; membrane protein intracellular domain proteolysis; Notch receptor processing; Notch signaling pathway; positive regulation of apoptosis; positive regulation of catalytic activity; protein processing

Disease: Acne Inversa, Familial, 1