MYT1 (PKMYT1) Blocking Peptide | PKMYT1 blocking peptide

MYT1 (PKMYT1) Antibody (C-term) Blocking peptide

• Gene Names: PKMYT1; MYT1; PPP1R126
• Synonyms: MYT1 (PKMYT1); MYT1 (PKMYT1) Antibody (C-term) Blocking peptide; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; Myt1 kinase; PKMYT1; MYT1; PKMYT1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the c-terminal region of human PKMYT1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 499

• Cellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PKMYT1 blocking peptide

Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.

NCBI and Uniprot Product Information

• NCBI GI #: 55976573
• NCBI GeneID: 9088
• UniProt Accession #: Q99640; O14731; Q7LE24; Q8TCM9; B3KUN8; B4DXD4; D3DUA4; F8W164; I3L1V2
• Molecular Weight: 47,284 Da
• NCBI Official Full Name: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• NCBI Official Synonym Full Names: protein kinase, membrane associated tyrosine/threonine 1
• NCBI Official Symbol: PKMYT1
• NCBI Official Synonym Symbols: MYT1; PPP1R126
• NCBI Protein Information: membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• UniProt Protein Name: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• Protein Family: Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase
• UniProt Gene Name: PKMYT1
• UniProt Synonym Gene Names: MYT1
• UniProt Entry Name: PMYT1_HUMAN

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

Myt1: a dual specificity protein kinase of the WEE family. Preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Alternative splice-variant isoforms have been reported.

Protein type: Protein kinase, dual-specificity (non-receptor); Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Other group; WEE family

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: cytosol; endoplasmic reticulum; Golgi apparatus; membrane; nucleoplasm

Molecular Function: kinase activity; protein binding; protein kinase activity

Biological Process: G1/S transition of mitotic cell cycle; G2/M transition of mitotic cell cycle; mitosis; regulation of cell cycle; regulation of cyclin-dependent protein kinase activity; regulation of mitosis