Mylk3 blocking peptide

Mouse Mylk3 Antibody (N-term) Blocking peptide

• Gene Names: Mylk3; MLCK; D830007F02Rik
• Synonyms: Mylk3; Mouse Mylk3 Antibody (N-term) Blocking peptide; Myosin light chain kinase 3; Cardiac-MyBP-C-associated Ca/CaM kinase; Cardiac-MLCK; Mylk3 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of Mouse Mylk3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 795

• Cellular Location: Cytoplasm.
• Tissue Location: Restricted to cardiomyocytes (at protein level). Down-regulated in heart after experimental myocardial infarction at the protein level; no significant changes at the mRNA level.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Mylk3 blocking peptide

Kinase that phosphorylates MYL2 in vitro. Has been proposed to be calmodulin-dependent (PubMed:17885681), although MYL2 phosphorylation has also been observed in the presence or absence of calmodulin (PubMed:18202317). Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility.

NCBI and Uniprot Product Information

• NCBI GI #: 123791378
• NCBI GeneID: 213435
• UniProt Accession #: Q3UIZ8; Q3V3V0; Q8BWD1; B0LY41
• Molecular Weight: 78,790 Da
• NCBI Official Full Name: Myosin light chain kinase 3
• NCBI Official Synonym Full Names: myosin light chain kinase 3
• NCBI Official Symbol: Mylk3
• NCBI Official Synonym Symbols: MLCK; D830007F02Rik
• NCBI Protein Information: myosin light chain kinase 3
• UniProt Protein Name: Myosin light chain kinase 3
• Protein Family: Myosin light chain kinase
• UniProt Gene Name: Mylk3
• UniProt Synonym Gene Names: Cardiac-MLCK
• UniProt Entry Name: MYLK3_MOUSE

Uniprot Description

caMLCK: Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, CAMK; EC 2.7.11.18; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; MLCK family

Cellular Component: actin cytoskeleton; cytoplasm; cytosol

Molecular Function: calmodulin-dependent protein kinase activity; myosin light chain kinase activity

Biological Process: cardiac myofibril assembly; protein amino acid phosphorylation; regulation of vascular permeability during acute inflammatory response; sarcomere organization; sarcomerogenesis