MAD2L2 blocking peptide

MAD2L2 Blocking Peptide

Cat. Num. AAA9620993

• Gene Names: MAD2L2; REV7; MAD2B; POLZ2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: MAD2L2; MAD2L2 Blocking Peptide; Homolog of REV7 S cerevisiae; hREV7; MAD2 (mitotic arrest deficient yeast; homolog) like 2; MAD2 homolog; MAD2 like 2; MAD2 mitotic arrest deficient like 2; MAD2-like protein 2; MAD2B; Mad2l2; MD2L2_HUMAN; Mitotic Arrest Deficient 2 L2; Mitotic arrest deficient 2-like protein 2; Mitotic arrest deficient homolog like 2; Mitotic arrest deficient like 2 (yeast); Mitotic arrest deficient yeast homolog; Mitotic spindle assembly checkpoint protein MAD2B; Polymerase (DNA directed) zeta 2 accessory subunit; POLZ2; REV 7; REV7; REV7 homolog; Weakly similar to Mitotic MAD2 protein (S cerevisiae); MAD2L2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for MAD2L2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for MAD2L2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 187960079
• NCBI GeneID: 10459
• NCBI Accession #: NP_001120797.1
• NCBI GenBank Nucleotide #: NM_001127325.1
• UniProt Accession #: Q9UI95; Q5TGW7; Q9UNA7; Q9Y6I6; B3KNE3
• Molecular Weight: 24,334 Da
• NCBI Official Full Name: mitotic spindle assembly checkpoint protein MAD2B
• NCBI Official Synonym Full Names: MAD2 mitotic arrest deficient-like 2 (yeast)
• NCBI Official Symbol: MAD2L2
• NCBI Official Synonym Symbols: REV7; MAD2B; POLZ2
• NCBI Protein Information: mitotic spindle assembly checkpoint protein MAD2B; hREV7; REV7 homolog; MAD2-like protein 2; mitotic arrest deficient homolog-like 2; mitotic arrest deficient 2-like protein 2; polymerase (DNA-directed), zeta 2, accessory subunit; MAD2 (mitotic arrest def
• UniProt Protein Name: Mitotic spindle assembly checkpoint protein MAD2B
• Protein Family: Mitotic spindle assembly checkpoint protein
• UniProt Gene Name: MAD2L2
• UniProt Synonym Gene Names: MAD2B; REV7; MAD2-like protein 2; hREV7
• UniProt Entry Name: MD2L2_HUMAN

NCBI Description

The protein encoded by this gene is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. The encoded protein, which is similar to MAD2L1, is capable of interacting with ADAM9, ADAM15, REV1, and REV3 proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

MAD2L2: Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: nucleoplasm; anaphase-promoting complex; zeta DNA polymerase complex; cytoplasm; spindle; nucleus

Molecular Function: JUN kinase binding; protein binding

Biological Process: mitosis; transcription, DNA-dependent; negative regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; actin filament organization; negative regulation of transcription from RNA polymerase II promoter; DNA repair; positive regulation of peptidyl-serine phosphorylation; cell division; double-strand break repair; mitotic cell cycle spindle assembly checkpoint; DNA damage response, signal transduction resulting in transcription; regulation of cell growth; negative regulation of protein catabolic process