KR1_VZVD blocking peptide

KR1_VZVD Blocking Peptide

Cat. Num. AAA9627808

• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: KR1_VZVD; KR1_VZVD Blocking Peptide; KR1_VZVD blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for KR1_VZVD blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for KR1_VZVD blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 9625940
• NCBI GeneID: 1487703
• NCBI Accession #: NP_040188.1
• NCBI GenBank Nucleotide #: NC_001348.1
• UniProt Accession #: P09251; CAA27949
• Molecular Weight: 43,680 Da
• NCBI Official Full Name: serine/threonine protein kinase US3
• NCBI Official Symbol: ORF66
• NCBI Protein Information: tegument protein; phosphorylates nuclear egress lamina protein; mediates phosphorylation of HDAC1 and HDAC2 and other cellular and viral proteins; serine/threonine protein kinase US3
• UniProt Protein Name: Serine/threonine-protein kinase US3 homolog
• UniProt Gene Name: 66
• UniProt Entry Name: US03_VZVD

Uniprot Description

Function: Multifunctional serine/threonine kinase that plays a role in several processes including egress of virus particles from the nucleus, modulation of the actin cytoskeleton and inhibition of apoptosis. Phosphorylates proteins 24 and 27, two critical regulators of capsid budding from nucleus to endoplasmic reticulum, thereby facilitating virion egress. Modulates and redistributes host components of the nuclear envelope, including LMNA, emerin/EMD and the nuclear matrix protein MATR3. Phosphorylates envelope glycoprotein B (gB), probably to direct it to the cell surface. Promotes virus intracellular spread by restructuring host cell cytoskeleton. Blocks host apoptosis to extend cell survival and allow efficient viral replication. Promotes viral gene expression by phosphorylating host HDAC2 to reduce viral genome silencing

By similarity. Downregulates class I major histocompatibility complex (MHC-I) surface expression. Additionally, phosphorylates IE62 and targets it to the cytoplasm. The nuclear exclusion of IE62 enables the packaging of abundant levels of IE62 into virions. Ref.5 Ref.6 Ref.7 Ref.8

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Subcellular location: Host cytoplasm

By similarity. Host nucleus

By similarity.

Post-translational modification: Phosphorylated by ORF47; this phosphorylation regulates subsequent phosphorylation of proteins 24 and 27 by ORF66. Autophosphorylated

By similarity. Ref.4 Ref.8

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Contains 1 protein kinase domain.