KDELR3 blocking peptide

KDELR3 Blocking Peptide

Cat. Num. AAA9624220

• Gene Names: KDELR3; ERD2L3
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: KDELR3; KDELR3 Blocking Peptide; ER lumen protein retaining receptor 3; ERD2L3; KDEL (Lys Asp Glu Leu) endoplasmic reticulum protein retention receptor 3; KDEL endoplasmic reticulum protein retention receptor 3; KDEL receptor 3; KDELR 3; KDELR3 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for KDELR3 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for KDELR3 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 6857802
• NCBI GeneID: 11015
• NCBI Accession #: NP_006846.1
• NCBI GenBank Nucleotide #: NM_006855.3
• UniProt Accession #: O43731; O95557; Q4V750; Q4V767; Q53FP4; Q53GK1; A8K7T7; B8ZZ26
• Molecular Weight: 25,027 Da
• NCBI Official Full Name: ER lumen protein retaining receptor 3 isoform a
• NCBI Official Synonym Full Names: KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 3
• NCBI Official Symbol: KDELR3
• NCBI Official Synonym Symbols: ERD2L3
• NCBI Protein Information: ER lumen protein retaining receptor 3; KDEL receptor 3; KDEL endoplasmic reticulum protein retention receptor 3
• UniProt Protein Name: ER lumen protein retaining receptor 3
• Protein Family: ER lumen protein-retaining receptor
• UniProt Gene Name: KDELR3
• UniProt Synonym Gene Names: KDEL receptor 3
• UniProt Entry Name: ERD23_HUMAN

NCBI Description

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. KDELR3 was the third member of the family to be identified, and it encodes a protein highly homologous to KDELR1 and KDELR2 proteins. Two transcript variants of KDELR3 that arise by alternative splicing, and encode different isoforms of KDELR3 receptor, have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

KDELR3: Required for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L. Belongs to the ERD2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, multi-pass; Endoplasmic reticulum; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: ER retention sequence binding

Biological Process: vesicle-mediated transport; protein transport; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; unfolded protein response; protein retention in ER