INSIG1 blocking peptide

INSIG1 Antibody (N-term) Blocking Peptide

• Gene Names: INSIG1; CL6; CL-6
• Synonyms: INSIG1; INSIG1 Antibody (N-term) Blocking Peptide; Insulin-induced gene 1 protein; INSIG-1; INSIG1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human INSIG1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 277

• Cellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein
• Tissue Location: Expressed in all tissues tested with highest expression in the liver.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for INSIG1 blocking peptide

Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth.

NCBI and Uniprot Product Information

• NCBI GI #: 115502405
• NCBI GeneID: 3638
• UniProt Accession #: O15503; Q53XW8; Q9BUV5; A4D2N1; A8K6L0
• Molecular Weight: 17,530 Da
• NCBI Official Full Name: Insulin-induced gene 1 protein
• NCBI Official Synonym Full Names: insulin induced gene 1
• NCBI Official Symbol: INSIG1
• NCBI Official Synonym Symbols: CL6; CL-6
• NCBI Protein Information: insulin-induced gene 1 protein
• UniProt Protein Name: Insulin-induced gene 1 protein
• Protein Family: Insulin-induced gene 1 protein
• UniProt Gene Name: INSIG1
• UniProt Synonym Gene Names: INSIG-1
• UniProt Entry Name: INSI1_HUMAN

NCBI Description

Oxysterols regulate cholesterol homeostasis through the liver X receptor (LXR)- and sterol regulatory element-binding protein (SREBP)-mediated signaling pathways. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jun 2009]

Uniprot Description

INSIG1: Mediates feedback control of cholesterol synthesis by controlling SCAP and HMGCR. Functions by blocking the processing of sterol regulatory element-binding proteins (SREBPs). Capable of retaining the SCAP-SREBF2 complex in the ER thus preventing it from escorting SREBPs to the Golgi. Initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase, AMFR/gp78. May play a role in growth and differentiation of tissues involved in metabolic control. May play a regulatory role during G0/G1 transition of cell growth. Belongs to the INSIG family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane

Molecular Function: protein binding

Biological Process: cell proliferation; metabolic process; SREBP-mediated signaling pathway