ILK1/ILK2 Blocking Peptide | Ilk blocking peptide

ILK1/ILK2 Antibody (N-term) Blocking Peptide

• Gene Names: Ilk; ESTM24; AA511515
• Synonyms: ILK1/ILK2; ILK1/ILK2 Antibody (N-term) Blocking Peptide; Integrin-linked protein kinase; Ilk; Ilk blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human ILK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 452

• Cellular Location: Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, myofibril, sarcomere. Cell projection, lamellipodium
• Tissue Location: Highly expressed in lung, heart, kidney, liver, brain, spleen and skeletal muscle. Weakly expressed in testis
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Ilk blocking peptide

Receptor-proximal protein kinase regulating integrin- mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 118595638
• NCBI GeneID: 16202
• UniProt Accession #: O55222; Q78KK2
• Molecular Weight: 51,373 Da
• NCBI Official Full Name: Integrin-linked protein kinase
• NCBI Official Synonym Full Names: integrin linked kinase
• NCBI Official Symbol: Ilk
• NCBI Official Synonym Symbols: ESTM24; AA511515
• NCBI Protein Information: integrin-linked protein kinase
• UniProt Protein Name: Integrin-linked protein kinase
• Protein Family: Integrin-linked protein kinase
• UniProt Gene Name: Ilk
• UniProt Entry Name: ILK_MOUSE

Uniprot Description

ILK: a tyrosine kinase-like kinase of the MLK family. Couples integrins and growth factors to downstream pathways involved in cell survival, cell cycle control, cell-cell adhesion and cell motility. Functions as a scaffold bridging the extra-cellular matrix and growth factor receptors to the actin cytoskeleton through interactions with the ILK-PINCH complex. This complex, which includes integrin, PINCH (which links ILK to receptor tyrosine kinases via Nck2), PARVA and affixin, is considered to be one of the convergence points of integrin- and growth factor-signaling pathways. May be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Stimulated downstream of PI3 kinase. Increased expression is correlated with progression of several tumor types, including breast, prostate, and colon carcinomas. Overexpression drives anchorage-independent growth and faster cell cycle.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; ILK subfamily

Cellular Component: axon; cell junction; cell soma; costamere; cytoplasm; dendritic shaft; focal adhesion; intercellular junction; lamellipodium; membrane; nucleoplasm; protein complex; stress fiber; terminal button

Molecular Function: integrin binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity; SH3 domain binding; signal transducer activity

Biological Process: cell aging; cell cycle arrest; cell projection organization and biogenesis; establishment and/or maintenance of epithelial cell polarity; fibril organization and biogenesis; integrin-mediated signaling pathway; myelin formation; myelination in the peripheral nervous system; negative regulation of apoptosis; negative regulation of neuron apoptosis; negative regulation of protein kinase activity; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; nerve development; neurite morphogenesis; peptidyl-serine phosphorylation; positive regulation of axon extension; positive regulation of axonogenesis; positive regulation of BMP signaling pathway; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of dendrite morphogenesis; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of myoblast differentiation; positive regulation of osteoblast differentiation; positive regulation of phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; protein heterooligomerization; protein kinase B signaling cascade; regulation of actin cytoskeleton organization and biogenesis; regulation of cell growth; Schwann cell development; ureteric bud branching