HTRA2 blocking peptide

HTRA2 Antibody (N-term) Blocking peptide

• Gene Names: HTRA2; OMI; PARK13; PRSS25
• Synonyms: HTRA2; HTRA2 Antibody (N-term) Blocking peptide; Serine protease HTRA2; mitochondrial; High temperature requirement protein A2; HtrA2; Omi stress-regulated endoprotease; Serine protease 25; Serine proteinase OMI; OMI; PRSS25; HTRA2 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of HTRA2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 458

• Cellular Location: Mitochondrion intermembrane space. Mitochondrion membrane; Single-pass membrane protein. Note: Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID
• Tissue Location: Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for HTRA2 blocking peptide

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.

NCBI and Uniprot Product Information

• NCBI GI #: 17376879
• NCBI GeneID: 27429
• UniProt Accession #: O43464; Q9HBZ4; Q9P0Y3; Q9P0Y4
• Molecular Weight: 39,914 Da
• NCBI Official Full Name: Serine protease HTRA2, mitochondrial
• NCBI Official Synonym Full Names: HtrA serine peptidase 2
• NCBI Official Symbol: HTRA2
• NCBI Official Synonym Symbols: OMI; PARK13; PRSS25
• NCBI Protein Information: serine protease HTRA2, mitochondrial
• UniProt Protein Name: Serine protease HTRA2, mitochondrial
• Protein Family: Serine protease
• UniProt Gene Name: HTRA2
• UniProt Synonym Gene Names: OMI; PRSS25; HtrA2
• UniProt Entry Name: HTRA2_HUMAN

NCBI Description

This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]

Uniprot Description

HTRA2: a serine proteinase that normally resides in mitochondrial membranes. It exists in two populations in mitochondria: as an unprocessed form probably attached to the inner mitochondrial membrane through a N-terminal transmembrane domain, and as a processed form containing a reaper motif at its N-terminus. Following apoptotic stimuli it is autoproteolytically activated and released into the cytosol, where it promotes programmed cell death in caspase-dependent and -independent manners. The amino-terminal reaper motif binds to the IAP (inhibitor of apoptosis) proteins cIAP1, cIAP2, and and XIAP, disrupting IAP-caspase complexes in a manner similar to Smac/DIABLO. Phosphorylation by the protein kinase Akt attenuates its serine protease and pro-apoptotic activities. The PDZ domain mediates interaction with Mxi2, an alternatively spliced form of the p38 stress-activated kinase. In contrast to its pro-apoptotic effects, targeted deletion in mice indicates that it is involved in protection against cell stress in striatial neurons. Defects in HTRA2 are the cause of Parkinson disease 13 (PARK13). Four alternatively spliced human isoforms have been described.

Protein type: Protease; Mitochondrial; Membrane protein, integral; EC 3.4.21.108

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: chromatin; cytoskeleton; cytosol; endoplasmic reticulum membrane; internal side of plasma membrane; membrane; mitochondrial intermembrane space; mitochondrion; nucleus

Molecular Function: peptidase activity; protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: cellular protein catabolic process; DNA damage response, signal transduction resulting in induction of apoptosis; negative regulation of cell cycle; positive regulation of apoptosis; positive regulation of caspase activity; protein autoprocessing; proteolysis

Disease: Parkinson Disease 13, Autosomal Dominant, Susceptibility To