Hck/Lyn blocking peptide

Phospho-Hck/Lyn (Tyr410/Tyr397) Blocking Peptide

Cat. Num. AAA9616084

• Gene Names: HCK; JTK9; p59Hck; p61Hck
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: Hck/Lyn; Phospho-Hck/Lyn (Tyr410/Tyr397) Blocking Peptide; AA407514; EC 2.7.10.2; FLJ26625; Hck 2; JTK 8; JTK8; Lck/Yes related novel protein tyrosine kinase; LYN; LYN proto oncogene; Src family tyrosine kinase; LYN_HUMAN; ONCOGENE LYN; p53Lyn; p56Lyn; Tyrosine protein kinase LYN; Tyrosine-protein kinase Lyn; V yes 1 Yamaguchi sarcoma viral related oncogene homolog; Yamaguchi sarcoma viral (v yes 1) related oncogene homolog; Bmk; Hck 1; Hck; HCK_HUMAN; Hemopoietic cell kinase; JTK9; p59-HCK/p60-HCK; p59Hck; p59HCK/p60HCK; p61Hck; Tyrosine protein kinase HCK; Tyrosine-protein kinase HCK; Hck/Lyn blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for Hck/Lyn blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for Hck/Lyn blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 287326139
• NCBI GeneID: 3055
• NCBI Accession #: NP_001165600.1
• NCBI GenBank Nucleotide #: NM_001172129.1
• UniProt Accession #: P08631; P07948
• Molecular Weight: 59,600 Da
• NCBI Official Full Name: tyrosine-protein kinase HCK isoform b
• NCBI Official Synonym Full Names: hemopoietic cell kinase
• NCBI Official Symbol: HCK
• NCBI Official Synonym Symbols: JTK9; p59Hck; p61Hck
• NCBI Protein Information: tyrosine-protein kinase HCK; p59-HCK/p60-HCK; hematopoietic cell kinase
• UniProt Protein Name: Tyrosine-protein kinase HCK
• UniProt Gene Name: HCK
• UniProt Entry Name: HCK_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010]

Uniprot Description

Hck: a protein-tyrosine kinase of the Src family that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms that have different subcellular localization.

Protein type: Oncoprotein; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; EC 2.7.10.2; Kinase, protein; TK group; Src family

Chromosomal Location of Human Ortholog: 20q11-q12

Cellular Component: Golgi apparatus; extrinsic to internal side of plasma membrane; transport vesicle; focal adhesion; cell projection; lysosome; actin filament; caveola; nucleus; cytosol

Molecular Function: protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: peptidyl-tyrosine phosphorylation; respiratory burst after phagocytosis; viral reproduction; protein amino acid autophosphorylation; regulation of phagocytosis; protein amino acid phosphorylation; regulation of cell shape; leukocyte migration during immune response; regulation of transcription factor activity; leukocyte degranulation; positive regulation of cell proliferation; lipopolysaccharide-mediated signaling pathway; mesoderm development; inflammatory response; innate immune response-activating signal transduction; cell adhesion; cell differentiation; integrin-mediated signaling pathway; cytokine and chemokine mediated signaling pathway; regulation of defense response to virus by virus; positive regulation of actin filament polymerization; regulation of inflammatory response; innate immune response; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis