GEM blocking peptide

GEM Blocking Peptide

Cat. Num. AAA9622457

• Gene Names: GEM; KIR
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: GEM; GEM Blocking Peptide; GTP binding mitogen induced T cell protein; GTP binding protein expressed in mitogen stimulated T cells; GTP binding protein GEM; GTP binding protein overexpressed in skeletal muscle; Kinase inducible Ras like protein; KIR; MGC26294; RAS like protein KIR; GEM blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for GEM blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for GEM blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4885263
• NCBI GeneID: 2669
• NCBI Accession #: NP_005252.1
• NCBI GenBank Nucleotide #: NM_005261.3
• UniProt Accession #: P55040; B2RA31
• Molecular Weight: 33,949 Da
• NCBI Official Full Name: GTP-binding protein GEM
• NCBI Official Synonym Full Names: GTP binding protein overexpressed in skeletal muscle
• NCBI Official Symbol: GEM
• NCBI Official Synonym Symbols: KIR
• NCBI Protein Information: GTP-binding protein GEM
• UniProt Protein Name: GTP-binding protein GEM
• Protein Family: Geminin
• UniProt Gene Name: GEM
• UniProt Synonym Gene Names: KIR

NCBI Description

The protein encoded by this gene belongs to the RAD/GEM family of GTP-binding proteins. It is associated with the inner face of the plasma membrane and could play a role as a regulatory protein in receptor-mediated signal transduction. Alternative splicing occurs at this locus and two transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

GEM: Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity. Belongs to the small GTPase superfamily. RGK family.

Protein type: G protein; G protein, monomeric; G protein, monomeric, RGK

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: internal side of plasma membrane; midbody; spindle midzone

Molecular Function: GDP binding; GTP binding; GTPase activity; magnesium ion binding; protein binding

Biological Process: cell surface receptor linked signal transduction; chromosome organization and biogenesis; immune response; metaphase plate congression; mitosis; signal transduction