FZD8 blocking peptide

FZD8 Blocking Peptide

Cat. Num. AAA9625100

• Gene Names: FZD8; FZ-8; hFZ8
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: FZD8; FZD8 Blocking Peptide; Frizzled 8 seven transmembrane spanning receptor; frizzled 8; seven transmembrane spanning receptor; Frizzled family receptor 8; frizzled homolog 8 (Drosophila); Frizzled homolog 8; Frizzled-8; FZ 8; Fz-8; FZ8; FZD 8; FZD8_HUMAN; hFZ 8; hFz8; HGNC4046; Homolog of Drosophila Frizzled 8; FZD8 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for FZD8 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for FZD8 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 13994190
• NCBI GeneID: 8325
• NCBI Accession #: NP_114072.1
• NCBI GenBank Nucleotide #: NM_031866.2
• UniProt Accession #: Q9H461
• Molecular Weight: 73,300 Da
• NCBI Official Full Name: frizzled-8
• NCBI Official Synonym Full Names: frizzled family receptor 8
• NCBI Official Symbol: FZD8
• NCBI Official Synonym Symbols: FZ-8; hFZ8
• NCBI Protein Information: frizzled-8; frizzled homolog 8; frizzled 8, seven transmembrane spanning receptor
• UniProt Protein Name: Frizzled-8
• Protein Family: Frizzled
• UniProt Gene Name: FZD8
• UniProt Synonym Gene Names: Fz-8; hFz8
• UniProt Entry Name: FZD8_HUMAN

NCBI Description

This intronless gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. This gene is highly expressed in two human cancer cell lines, indicating that it may play a role in several types of cancer. The crystal structure of the extracellular cysteine-rich domain of a similar mouse protein has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD8: Receptor for Wnt proteins. Component of the Wnt-Fzd- LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome- sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, Fz/Smo family

Chromosomal Location of Human Ortholog: 10p11.21

Cellular Component: Golgi apparatus; plasma membrane; integral to membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding; ubiquitin protein ligase binding; PDZ domain binding; receptor binding

Biological Process: neuron differentiation; positive regulation of JNK activity; G-protein coupled receptor protein signaling pathway; T cell differentiation in the thymus; positive regulation of transcription from RNA polymerase II promoter; angiogenesis; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin