FER blocking peptide

FER Blocking Peptide

Cat. Num. AAA9619810

• Gene Names: FER; TYK3; PPP1R74; p94-Fer
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: FER; FER Blocking Peptide; c FER; Feline encephalitis virus related kinase FER; Fer (fps/fes related) tyrosine kinase (phosphoprotein NCP94); Fer (fps/fes related) tyrosine kinase; FER_HUMAN; FerT; Fujinami poultry sarcoma/Feline sarcoma related protein Fer; p94 FER; p94-FER; Pe1Fe10; Pe1Fe13; Pe1Fe3; Pe1Fe6; Phosphoprotein NCP94; PPP1R74; Protein phosphatase 1 regulatory subunit 74; Proto oncogene tyrosine protein kinase FER; Proto-oncogene c-Fer; TYK3; Tyrosine kinase 3; Tyrosine-protein kinase Fer; FER blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for FER blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for FER blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 808392675
• NCBI GeneID: 2241
• NCBI Accession #: NP_001294957.1
• NCBI GenBank Nucleotide #: NM_001308028.1
• UniProt Accession #: P16591; B2RCR4; B4DSQ2; H2FLB8
• Molecular Weight: 51,643 Da
• NCBI Official Full Name: tyrosine-protein kinase Fer isoform b
• NCBI Official Synonym Full Names: FER tyrosine kinase
• NCBI Official Symbol: FER
• NCBI Official Synonym Symbols: TYK3; PPP1R74; p94-Fer
• NCBI Protein Information: tyrosine-protein kinase Fer
• UniProt Protein Name: Tyrosine-protein kinase Fer
• Protein Family: Ferritin
• UniProt Gene Name: FER
• UniProt Synonym Gene Names: TYK3
• UniProt Entry Name: FER_HUMAN

NCBI Description

The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome X. [provided by RefSeq, Apr 2015]

Uniprot Description

Fer: a non-receptor tyrosine kinase of the Fer family. Regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Increased expression associated with prostate cancer proliferation, and antisense blocks soft agar growth.

Protein type: Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); Oncoprotein; EC 2.7.10.2; TK group; Fer family

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: microtubule cytoskeleton; extrinsic to internal side of plasma membrane; lamellipodium; cytoplasm; nuclear chromatin; cell cortex; cytosol; nucleus; cell junction; actin cytoskeleton

Molecular Function: protein binding; cadherin binding; gamma-catenin binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; actin binding; lipid binding; protein kinase binding; protein phosphatase 1 binding; Rab GTPase binding; ATP binding; epidermal growth factor receptor binding

Biological Process: regulation of mast cell degranulation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; diapedesis; tyrosine phosphorylation of Stat3 protein; response to lipopolysaccharide; platelet-derived growth factor receptor signaling pathway; negative regulation of mast cell activation during immune response; chemotaxis; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; positive regulation of cell proliferation; cell adhesion; cell differentiation; regulation of protein amino acid phosphorylation; cytokine and chemokine mediated signaling pathway; microtubule cytoskeleton organization and biogenesis; regulation of epidermal growth factor receptor signaling pathway; regulation of cell proliferation; cell proliferation; positive regulation of actin filament polymerization; cellular response to insulin stimulus; actin cytoskeleton reorganization; innate immune response; mitotic cell cycle; positive regulation of cell migration