FER blocking peptide

FER Antibody (C-term) Blocking Peptide

• Gene Names: FER; TYK3; PPP1R74; p94-Fer
• Synonyms: FER; FER Antibody (C-term) Blocking Peptide; Tyrosine-protein kinase Fer; Feline encephalitis virus-related kinase FER; Fujinami poultry sarcoma/Feline sarcoma-related protein Fer; Proto-oncogene c-Fer; Tyrosine kinase 3; p94-Fer; TYK3; FER blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human FER. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 822

• Cellular Location: Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell junction. Membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Cytoplasm, cell cortex. Note: Associated with the chromatin. Detected on microtubules in polarized and motile vascular endothelial cells. Colocalizes with F-actin at the cell cortex. Colocalizes with PECAM1 and CTNND1 at nascent cell- cell contacts
• Tissue Location: Isoform 1 is detected in normal colon and in fibroblasts (at protein level). Isoform 3 is detected in normal testis, in colon carcinoma-derived metastases in lung, liver and ovary, and in colon carcinoma and hepato carcinoma cell lines (at protein level). Isoform 3 is not detected in normal colon or in normal fibroblasts (at protein level). Widely expressed
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for FER blocking peptide

Tyrosine-protein kinase that acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. Acts downstream of EGFR, KIT, PDGFRA and PDGFRB. Acts downstream of EGFR to promote activation of NF-kappa-B and cell proliferation. May play a role in the regulation of the mitotic cell cycle. Plays a role in the insulin receptor signaling pathway and in activation of phosphatidylinositol 3-kinase. Acts downstream of the activated FCER1 receptor and plays a role in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Plays a role in the regulation of mast cell degranulation. Plays a role in leukocyte recruitment and diapedesis in response to bacterial lipopolysaccharide (LPS). Plays a role in synapse organization, trafficking of synaptic vesicles, the generation of excitatory postsynaptic currents and neuron-neuron synaptic transmission. Plays a role in neuronal cell death after brain damage. Phosphorylates CTTN, CTNND1, PTK2/FAK1, GAB1, PECAM1 and PTPN11. May phosphorylate JUP and PTPN1. Can phosphorylate STAT3, but the biological relevance of this depends on cell type and stimulus.

NCBI and Uniprot Product Information

• NCBI GI #: 97536202
• NCBI GeneID: 2241
• UniProt Accession #: P16591; B2RCR4; B4DSQ2; H2FLB8
• Molecular Weight: 51,643 Da
• NCBI Official Full Name: Tyrosine-protein kinase Fer
• NCBI Official Synonym Full Names: FER tyrosine kinase
• NCBI Official Symbol: FER
• NCBI Official Synonym Symbols: TYK3; PPP1R74; p94-Fer
• NCBI Protein Information: tyrosine-protein kinase Fer
• UniProt Protein Name: Tyrosine-protein kinase Fer
• Protein Family: Ferritin
• UniProt Gene Name: FER
• UniProt Synonym Gene Names: TYK3
• UniProt Entry Name: FER_HUMAN

NCBI Description

The protein encoded by this gene is a member of the FPS/FES family of non-transmembrane receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome X. [provided by RefSeq, Apr 2015]

Uniprot Description

Fer: a non-receptor tyrosine kinase of the Fer family. Regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. Increased expression associated with prostate cancer proliferation, and antisense blocks soft agar growth.

Protein type: Kinase, protein; Protein kinase, TK; Oncoprotein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Fer family

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: actin cytoskeleton; cell junction; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; lamellipodium; microtubule cytoskeleton; nuclear chromatin; nucleus

Molecular Function: epidermal growth factor receptor binding; lipid binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein-tyrosine kinase activity

Biological Process: actin cytoskeleton reorganization; activation of NF-kappaB transcription factor; cell adhesion; cell differentiation; cell proliferation; cellular response to insulin stimulus; chemotaxis; cytokine and chemokine mediated signaling pathway; diapedesis; innate immune response; microtubule cytoskeleton organization and biogenesis; mitotic cell cycle; negative regulation of mast cell activation during immune response; peptidyl-tyrosine phosphorylation; platelet-derived growth factor receptor signaling pathway; positive regulation of actin filament polymerization; positive regulation of cell migration; positive regulation of cell proliferation; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of epidermal growth factor receptor signaling pathway; regulation of mast cell degranulation; regulation of protein amino acid phosphorylation; response to lipopolysaccharide; tyrosine phosphorylation of Stat3 protein