FBLIM1 blocking peptide

FBLIM1 Blocking Peptide

Cat. Num. AAA9621708

• Gene Names: FBLIM1; CAL; FBLP1; FBLP-1
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: FBLIM1; FBLIM1 Blocking Peptide; 2410043F08Rik; Cal; CSX associated LIM; DKFZp434G171; FBLI1_HUMAN; FBLIM 1; Fblim1; FBLP 1; FBLP-1; FBLP1; Filamin binding LIM protein 1; Filamin-binding LIM protein 1; Gt10; MGC105665; MIG2 interacting protein; MIG2-interacting protein; Migfilin; Mitogen inducible 2 interacting protein; Mitogen-inducible 2-interacting protein; OTTHUMP00000003118; OTTHUMP00000003119; OTTHUMP00000003120; OTTMUSP00000011062; OTTMUSP00000011063; RP11-169K16.5; RP23-291H3.1; FBLIM1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for FBLIM1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for FBLIM1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 66932988
• NCBI GeneID: 54751
• NCBI Accession #: NP_001019387.1
• NCBI GenBank Nucleotide #: NP_001019387.1
• UniProt Accession #: Q8WUP2; Q5VVE0; Q5VVE1; Q8IX23; Q96T00; Q9UFD6; B3KNY0
• Molecular Weight: 40,670 Da
• NCBI Official Full Name: filamin-binding LIM protein 1 isoform c
• NCBI Official Synonym Full Names: filamin binding LIM protein 1
• NCBI Official Symbol: FBLIM1
• NCBI Official Synonym Symbols: CAL; FBLP1; FBLP-1
• NCBI Protein Information: filamin-binding LIM protein 1; migfilin; CSX-associated LIM; MIG2-interacting protein; filamin-binding LIM protein-1; mitogen-inducible 2 interacting protein; mitogen-inducible 2-interacting protein
• UniProt Protein Name: Filamin-binding LIM protein 1
• Protein Family: Filamin-binding LIM protein
• UniProt Gene Name: FBLIM1
• UniProt Synonym Gene Names: FBLP1; FBLP-1
• UniProt Entry Name: FBLI1_HUMAN

NCBI Description

This gene encodes a protein with an N-terminal filamin-binding domain, a central proline-rich domain, and, multiple C-terminal LIM domains. This protein localizes at cell junctions and may link cell adhesion structures to the actin cytoskeleton. This protein may be involved in the assembly and stabilization of actin-filaments and likely plays a role in modulating cell adhesion, cell morphology and cell motility. This protein also localizes to the nucleus and may affect cardiomyocyte differentiation after binding with the CSX/NKX2-5 transcription factor. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

FBLIM1: Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin- containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: focal adhesion; stress fiber; cell cortex; cytosol

Molecular Function: zinc ion binding; filamin binding

Biological Process: regulation of cell shape; cell-cell adhesion; regulation of integrin activation