EXOSC8 blocking peptide

EXOSC8 Blocking Peptide

Cat. Num. AAA9621308

• Gene Names: EXOSC8; p9; CIP3; EAP2; OIP2; PCH1C; RRP43; Rrp43p; bA421P11.3
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: EXOSC8; EXOSC8 Blocking Peptide; bA421P11.3; CBP interacting protein 3; CIP3; EAP2; EC 3.1.13; EXOS8_HUMAN; Exosome complex component RRP43; Exosome complex exonuclease RRP43; Exosome component 8; OIP-2; OIP2; Opa interacting protein 2; Opa-interacting protein 2; OTTHUMP00000042274; p9; Ribosomal RNA processing protein 43; S. cerevisiae; homolog of; Ribosomal RNA-processing protein 43; RP11 421P11.3; RRP43; Rrp43p; EXOSC8 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for EXOSC8 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EXOSC8 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 31415882
• NCBI GeneID: 11340
• NCBI Accession #: NP_852480
• NCBI GenBank Nucleotide #: NP_852480.1
• UniProt Accession #: Q96B26; O43480; Q5TBA5
• Molecular Weight: 32.4 kDa (299aa) confirmed by MALDI-TOF
• NCBI Official Full Name: exosome complex component RRP43
• NCBI Official Synonym Full Names: exosome component 8
• NCBI Official Symbol: EXOSC8
• NCBI Official Synonym Symbols: p9; CIP3; EAP2; OIP2; PCH1C; RRP43; Rrp43p; bA421P11.3
• NCBI Protein Information: exosome complex component RRP43
• UniProt Protein Name: Exosome complex component RRP43
• Protein Family: Exosome complex component
• UniProt Gene Name: EXOSC8
• UniProt Synonym Gene Names: OIP2; RRP43; OIP-2

NCBI Description

This gene encodes a 3'-5' exoribonuclease that specifically interacts with mRNAs containing AU-rich elements. The encoded protein is part of the exosome complex that is important for the degradation of numerous RNA species. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Mar 2009]

Uniprot Description

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC8 binds to ARE-containing RNAs.