EXOSC8 blocking peptide

EXOSC8 Antibody (C-term) Blocking Peptide

• Gene Names: EXOSC8; p9; CIP3; EAP2; OIP2; PCH1C; RRP43; Rrp43p; bA421P11.3
• Synonyms: EXOSC8; EXOSC8 Antibody (C-term) Blocking Peptide; Exosome complex component RRP43; Exosome component 8; Opa-interacting protein 2; OIP-2; Ribosomal RNA-processing protein 43; p9; OIP2; RRP43; EXOSC8 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human EXOSC8. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 276

• Cellular Location: Cytoplasm. Nucleus. Nucleus, nucleolus
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EXOSC8 blocking peptide

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC8 binds to ARE-containing RNAs.

NCBI and Uniprot Product Information

• NCBI GI #: 21759409
• NCBI GeneID: 11340
• UniProt Accession #: Q96B26; O43480; Q5TBA5
• Molecular Weight: 30,040 Da
• NCBI Official Full Name: Exosome complex component RRP43
• NCBI Official Synonym Full Names: exosome component 8
• NCBI Official Symbol: EXOSC8
• NCBI Official Synonym Symbols: p9; CIP3; EAP2; OIP2; PCH1C; RRP43; Rrp43p; bA421P11.3
• NCBI Protein Information: exosome complex component RRP43
• UniProt Protein Name: Exosome complex component RRP43
• Protein Family: Exosome complex component
• UniProt Gene Name: EXOSC8
• UniProt Synonym Gene Names: OIP2; RRP43; OIP-2
• UniProt Entry Name: EXOS8_HUMAN

NCBI Description

This gene encodes a 3'-5' exoribonuclease that specifically interacts with mRNAs containing AU-rich elements. The encoded protein is part of the exosome complex that is important for the degradation of numerous RNA species. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Mar 2009]

Uniprot Description

EXOSC8: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC8 binds to ARE-containing RNAs. Belongs to the RNase PH family.

Protein type: Ribonuclease; EC 3.1.13.-; Nucleolus

Chromosomal Location of Human Ortholog: 13q13.1

Cellular Component: cytoplasm; cytosol; exosome (RNase complex); nuclear exosome (RNase complex); nucleoplasm; nucleus

Molecular Function: AU-rich element binding; exoribonuclease activity; protein binding

Biological Process: exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); regulation of mRNA stability; rRNA processing

Disease: Pontocerebellar Hypoplasia, Type 1c