EPHA3 blocking peptide

EphA3 Antibody (C-term) Blocking Peptide

• Gene Names: EPHA3; EK4; ETK; HEK; ETK1; HEK4; TYRO4
• Synonyms: EphA3; EphA3 Antibody (C-term) Blocking Peptide; Ephrin type-A receptor 3; EPH-like kinase 4; EK4; hEK4; HEK; Human embryo kinase; Tyrosine-protein kinase TYRO4; Tyrosine-protein kinase receptor ETK1; Eph-like tyrosine kinase 1; EPHA3; ETK; ETK1; TYRO4; EPHA3 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human EphA3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 983

• Cellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein
• Tissue Location: Widely expressed. Highest level in placenta.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EPHA3 blocking peptide

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous for ephrin-A ligands it binds preferentially EFNA5. Upon activation by EFNA5 regulates cell-cell adhesion, cytoskeletal organization and cell migration. Plays a role in cardiac cells migration and differentiation and regulates the formation of the atrioventricular canal and septum during development probably through activation by EFNA1. Involved in the retinotectal mapping of neurons. May also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.

NCBI and Uniprot Product Information

• NCBI GI #: 116241351
• NCBI GeneID: 2042
• UniProt Accession #: P29320; Q9H2V3; Q9H2V4
• Molecular Weight: 60,946 Da
• NCBI Official Full Name: Ephrin type-A receptor 3
• NCBI Official Synonym Full Names: EPH receptor A3
• NCBI Official Symbol: EPHA3
• NCBI Official Synonym Symbols: EK4; ETK; HEK; ETK1; HEK4; TYRO4
• NCBI Protein Information: ephrin type-A receptor 3
• UniProt Protein Name: Ephrin type-A receptor 3
• Protein Family: Ephrin type-A receptor
• UniProt Gene Name: EPHA3
• UniProt Synonym Gene Names: ETK; ETK1; HEK; TYRO4; EK4; hEK4; Human embryo kinase; Eph-like tyrosine kinase 1
• UniProt Entry Name: EPHA3_HUMAN

NCBI Description

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

EphA3: a receptor tyrosine kinase of the Eph family. Receptor for members of the ephrin-A family: binds to ephrin-A2, -A3, -A4 and -A5The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Two point mutations seen in a survey of colorectal tumors. Soluble receptors reduce tumor growth and angiogenesis in mouse models. Two alternatively spliced isoforms of EphA3 have been described.

Protein type: Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; Eph family

Chromosomal Location of Human Ortholog: 3p11.2

Cellular Component: early endosome; integral to plasma membrane; plasma membrane

Molecular Function: GPI-linked ephrin receptor activity; protein binding

Biological Process: cell migration; ephrin receptor signaling pathway; regulation of actin cytoskeleton organization and biogenesis; regulation of focal adhesion formation; regulation of GTPase activity