EIF2S1 blocking peptide

EIF2S1 Antibody (N-term) Blocking peptide

• Gene Names: EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
• Synonyms: EIF2S1; EIF2S1 Antibody (N-term) Blocking peptide; Eukaryotic translation initiation factor 2 subunit 1; Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha; EIF2A; EIF2S1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of EIF2S1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 315

• Cellular Location: Cytoplasmic granule. Note: The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EIF2S1 blocking peptide

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

NCBI and Uniprot Product Information

• NCBI GI #: 124200
• NCBI GeneID: 1965
• UniProt Accession #: P05198
• Molecular Weight: 36,112 Da
• NCBI Official Full Name: Eukaryotic translation initiation factor 2 subunit 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2 subunit alpha
• NCBI Official Symbol: EIF2S1
• NCBI Official Synonym Symbols: EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
• NCBI Protein Information: eukaryotic translation initiation factor 2 subunit 1
• UniProt Protein Name: Eukaryotic translation initiation factor 2 subunit 1
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF2S1
• UniProt Synonym Gene Names: EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha
• UniProt Entry Name: IF2A_HUMAN

NCBI Description

The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]

Uniprot Description

eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.

Protein type: Translation initiation; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: cytosol; eukaryotic translation initiation factor 2 complex; eukaryotic translation initiation factor 2B complex; membrane; polysome; ribosome; stress granule

Molecular Function: protein binding; ribosome binding; translation initiation factor activity

Biological Process: cellular response to amino acid starvation; negative regulation of translation initiation in response to stress; positive regulation of defense response to virus by host; translational initiation; transmembrane transport