EIF2B3 blocking peptide

EIF2B3 Antibody (C-term) Blocking Peptide

• Gene Names: EIF2B3; EIF-2B; EIF2Bgamma
• Synonyms: EIF2B3; EIF2B3 Antibody (C-term) Blocking Peptide; Translation initiation factor eIF-2B subunit gamma; eIF-2B GDP-GTP exchange factor subunit gamma; EIF2B3 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human EIF2B3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Lyophilized with 100% acetonitrile and is supplied as a powder.
• Sequence Length: 412

• Reconstitution: Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for EIF2B3 blocking peptide

EIF2B3 catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Mutations in each of the five subunits of translation initiation factor eIF2B, including subunit 3, can cause leukoencephalopathy with vanishing white matter. EIF2B3 has also been identified as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation.

NCBI and Uniprot Product Information

• NCBI GI #: 262205273
• NCBI GeneID: 8891
• NCBI Accession #: NP_001160060.1; NP_001248347.1; NP_065098.1
• NCBI GenBank Nucleotide #: NM_001166588.2
• UniProt Accession #: Q9NR50; Q4R6T3; Q9HA31
• NCBI Official Full Name: translation initiation factor eIF-2B subunit gamma isoform 2
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 2B subunit gamma
• NCBI Official Symbol: EIF2B3
• NCBI Official Synonym Symbols: EIF-2B; EIF2Bgamma
• NCBI Protein Information: translation initiation factor eIF-2B subunit gamma
• UniProt Protein Name: Translation initiation factor eIF-2B subunit gamma
• Protein Family: Translation initiation factor
• UniProt Gene Name: EIF2B3
• UniProt Entry Name: EI2BG_HUMAN

NCBI Description

The protein encoded by this gene is one of the subunits of initiation factor eIF2B, which catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. It has also been found to function as a cofactor of hepatitis C virus internal ribosome entry site-mediated translation. Mutations in this gene have been associated with leukodystrophy with vanishing white matter. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

eIF2B-gamma: Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP. Defects in EIF2B3 are a cause of leukodystrophy with vanishing white matter (VWM). VWM is a leukodystrophy that occurs mainly in children. Neurological signs include progressive cerebellar ataxia, spasticity, inconstant optic atrophy and relatively preserved mental abilities. The disease is chronic-progressive with, in most individuals, additional episodes of rapid deterioration following febrile infections or minor head trauma. While childhood onset is the most common form of the disorder, some severe forms are apparent at birth. A severe, early-onset form seen among the Cree and Chippewayan populations of Quebec and Manitoba is called Cree leukoencephalopathy. Milder forms may not become evident until adolescence or adulthood. Some females with milder forms of the disease who survive to adolescence exhibit ovarian dysfunction. This variant of the disorder is called ovarioleukodystrophy. Belongs to the eIF-2B gamma/epsilon subunits family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: eukaryotic translation initiation factor 2B complex; cytoplasm; cytosol

Molecular Function: protein binding; translation factor activity, nucleic acid binding; guanyl-nucleotide exchange factor activity; translation initiation factor activity; nucleotidyltransferase activity

Biological Process: response to peptide hormone stimulus; cellular protein metabolic process; translation; response to heat; response to glucose stimulus; translational initiation; negative regulation of translation initiation in response to stress; cellular response to stimulus; gene expression; oligodendrocyte development; positive regulation of GTPase activity

Disease: Leukoencephalopathy With Vanishing White Matter