DOCK1 blocking peptide

DOCK1 Blocking Peptide

Cat. Num. AAA9624157

• Gene Names: DOCK1; ced5; DOCK180
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: DOCK1; DOCK1 Blocking Peptide; 180 kDa protein downstream of CRK; Ced5; Dedicator of Cytokinesis 1; Dedicator of cytokinesis protein 1; DOCK 1; DOCK 180; DOCK1_HUMAN; DOCK180; DOCK1 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for DOCK1 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DOCK1 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4503355
• NCBI GeneID: 1793
• NCBI Accession #: NP_001371.1
• NCBI GenBank Nucleotide #: NM_001380.4
• UniProt Accession #: Q14185; A9Z1Z5
• Molecular Weight: 215,346 Da
• NCBI Official Full Name: dedicator of cytokinesis protein 1
• NCBI Official Synonym Full Names: dedicator of cytokinesis 1
• NCBI Official Symbol: DOCK1
• NCBI Official Synonym Symbols: ced5; DOCK180
• NCBI Protein Information: dedicator of cytokinesis protein 1; DOwnstream of CrK; dedicator of cyto-kinesis 1; 180 kDa protein downstream of CRK
• UniProt Protein Name: Dedicator of cytokinesis protein 1
• Protein Family: Dedicator of cytokinesis protein
• UniProt Gene Name: DOCK1
• UniProt Synonym Gene Names: DOCK180
• UniProt Entry Name: DOCK1_HUMAN

NCBI Description

This gene product binds to the SH3 domain of CRK protein. It may regulate cell surface extension and may have a role in the cell surface extension of an engulfing cell around a dying cell during apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

DOCK1: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1. Interacts with the SH3 domains of CRK and NCK2 via multiple sites. Interacts with nucleotide-free RAC1 via its DHR-2 domain. Interacts with ELMO1, ELMO2 and probably ELMO3 via its SH3 domain. Interacts with RAC1 and BAI1. Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon. Belongs to the DOCK family.

Protein type: GEFs, Rac/Rho; Cytoskeletal; Motility/polarity/chemotaxis; Adaptor/scaffold; GEFs

Chromosomal Location of Human Ortholog: 10q26.13-q26.3

Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; guanyl-nucleotide exchange factor activity; SH3 domain binding; GTPase activator activity

Biological Process: integrin-mediated signaling pathway; axon guidance; cell migration; apoptosis; small GTPase mediated signal transduction; innate immune response; hemopoietic progenitor cell differentiation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; signal transduction; phagocytosis, engulfment; positive regulation of GTPase activity