DGKQ blocking peptide

DGKQ Antibody (C-term) Blocking Peptide

• Gene Names: DGKQ; DAGK; DAGK4; DAGK7
• Synonyms: DGKQ; DGKQ Antibody (C-term) Blocking Peptide; Diacylglycerol kinase theta; DAG kinase theta; Diglyceride kinase theta; DGK-theta; DAGK4; DGKQ blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human DGKQ. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 942

• Cellular Location: Cytoplasm. Cell membrane. Cytoplasm, cytoskeleton. Nucleus. Nucleus speckle. Note: Translocates to the nucleus in response to thrombin stimulation (By similarity) Translocates to the plasma membrane in response to steroid hormone receptor stimulation. Translocation to the plasma membrane is dependent on G-protein coupled receptor stimulation and subsequent activation of PRKCE and probably PRKCH.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for DGKQ blocking peptide

Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor (By similarity). May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity.

NCBI and Uniprot Product Information

• NCBI GI #: 257051005
• NCBI GeneID: 1609
• UniProt Accession #: P52824; Q6P3W4
• Molecular Weight: 101,155 Da
• NCBI Official Full Name: Diacylglycerol kinase theta
• NCBI Official Synonym Full Names: diacylglycerol kinase theta
• NCBI Official Symbol: DGKQ
• NCBI Official Synonym Symbols: DAGK; DAGK4; DAGK7
• NCBI Protein Information: diacylglycerol kinase theta
• UniProt Protein Name: Diacylglycerol kinase theta
• Protein Family: Diacylglycerol kinase
• UniProt Gene Name: DGKQ
• UniProt Synonym Gene Names: DAGK4; DAG kinase theta; DGK-theta
• UniProt Entry Name: DGKQ_HUMAN

NCBI Description

The protein encoded by this gene contains three cysteine-rich domains, a proline-rich region, and a pleckstrin homology domain with an overlapping Ras-associating domain. It is localized in the speckle domains of the nucleus, and mediates the regeneration of phosphatidylinositol (PI) from diacylglycerol in the PI-cycle during cell signal transduction. [provided by RefSeq, Jul 2008]

Uniprot Description

DGKQ: Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA). May regulate the activity of protein kinase C by controlling the balance between these two signaling lipids. Activated in the nucleus in response to alpha-thrombin and nerve growth factor. May be involved in cAMP- induced activation of NR5A1 and subsequent steroidogenic gene transcription by delivering PA as ligand for NR5A1. Acts synergistically with NR5A1 on CYP17 transcriptional activity. Belongs to the eukaryotic diacylglycerol kinase family.

Protein type: EC 2.7.1.107; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - glycerolipid; Motility/polarity/chemotaxis; Kinase, lipid

Chromosomal Location of Human Ortholog: 4p16.3

Cellular Component: cytoplasm; cytoskeleton; cytosol; nuclear speck; nucleus; plasma membrane

Molecular Function: diacylglycerol kinase activity; kinase binding; phospholipase binding; protein binding; transcription activator binding

Biological Process: cAMP-mediated signaling; G-protein coupled receptor protein signaling pathway; platelet activation; regulation of transcription from RNA polymerase II promoter; response to ATP