CFL1 blocking peptide

CFL1 Antibody (N-term) Blocking Peptide

• Gene Names: CFL1; CFL; cofilin; HEL-S-15
• Synonyms: CFL1; CFL1 Antibody (N-term) Blocking Peptide; Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform; CFL; CFL1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human CFL1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 166

• Cellular Location: Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Detected at the cleavage furrow and contractile ring during cytokinesis. Almost completely in nucleus in cells exposed to heat shock or 10% dimethyl sulfoxide
• Tissue Location: Widely distributed in various tissues.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CFL1 blocking peptide

Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation.

NCBI and Uniprot Product Information

• NCBI GI #: 116848
• NCBI GeneID: 1072
• UniProt Accession #: P23528; Q53Y87; Q9UCA2; B3KUQ1
• Molecular Weight: 18,502 Da
• NCBI Official Full Name: Cofilin-1
• NCBI Official Synonym Full Names: cofilin 1
• NCBI Official Symbol: CFL1
• NCBI Official Synonym Symbols: CFL; cofilin; HEL-S-15
• NCBI Protein Information: cofilin-1
• UniProt Protein Name: Cofilin-1
• Protein Family: Cofilin
• UniProt Gene Name: CFL1
• UniProt Synonym Gene Names: CFL; p18
• UniProt Entry Name: COF1_HUMAN

NCBI Description

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Uniprot Description

Cofilin-1: a cytoskeletal protein that controls actin depolymerization. Has a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1. May promote filament assembly rather than disassembly. Two alternatively spliced variants are described. Isoform b is expressed predominantly in skeletal muscle and heart, while isoform a is expressed in various tissues.

Protein type: Cytoskeletal; Nuclear receptor co-regulator; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: cytoplasm; extracellular space; focal adhesion; membrane; nucleus; vesicle

Molecular Function: protein binding

Biological Process: actin cytoskeleton organization and biogenesis; cytoskeleton organization and biogenesis; negative regulation of apoptosis; regulation of cell morphogenesis; response to virus; Rho protein signal transduction