CENPK blocking peptide

CENPK Blocking Peptide

Cat. Num. AAA9628080

• Gene Names: CENPK; P33; Solt; CENP-K; FKSG14; AF5alpha
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: CENPK; CENPK Blocking Peptide; AF5 alpha; AF5alpha; B130045K24Rik; BB232189; C530004N04Rik; CENP K; Centromere protein K; Centromeric protein K; FKSG 14; FKSG14; ICEN 37; ICEN37; Interphase centromere complex protein 37; Leucine zipper protein FKSG14; OTTHUMP00000161888; P33; Protein AF 5alpha; Protein AF5alpha; Solt; Solzt; SoxLZ/Sox6 binding protein Solt; SoxLZ/Sox6 leucine zipper binding protein; CENPK blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for CENPK blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CENPK blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 388490144
• NCBI GeneID: 64105
• NCBI Accession #: NP_001253967.1
• NCBI GenBank Nucleotide #: NM_001267038.1
• UniProt Accession #: Q9BS16; Q9H4L0
• Molecular Weight: 31,655 Da
• NCBI Official Full Name: centromere protein K
• NCBI Official Synonym Full Names: centromere protein K
• NCBI Official Symbol: CENPK
• NCBI Official Synonym Symbols: P33; Solt; CENP-K; FKSG14; AF5alpha
• NCBI Protein Information: centromere protein K; protein AF-5alpha; leucine zipper protein FKSG14; SoxLZ/Sox6-binding protein Solt; interphase centromere complex protein 37
• UniProt Protein Name: Centromere protein K
• Protein Family: Centromere protein
• UniProt Gene Name: CENPK
• UniProt Synonym Gene Names: ICEN37; CENP-K
• UniProt Entry Name: CENPK_HUMAN

NCBI Description

CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM, Mar 2008]

Uniprot Description

CENPK: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore. Chromosomal aberrations involving CENPK are a cause of acute leukemias. Translocation t(5;11)(q12;q23) with MLL. Belongs to the CENPK family.

Chromosomal Location of Human Ortholog: 5q12.3

Cellular Component: nucleoplasm; cytosol

Biological Process: nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle