BAP1 blocking peptide

BAP1 Antibody (N-term) Blocking Peptide

• Gene Names: BAP1; UCHL2; hucep-6; HUCEP-13
• Synonyms: BAP1; BAP1 Antibody (N-term) Blocking Peptide; Ubiquitin carboxyl-terminal hydrolase BAP1; BRCA1-associated protein 1; Cerebral protein 6; KIAA0272; BAP1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human BAP1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 729

• Cellular Location: Cytoplasm. Nucleus. Note: Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O (PubMed:24703950).
• Tissue Location: Highly expressed in testis, placenta and ovary. Expressed in breast.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for BAP1 blocking peptide

Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'- linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Able to mediate autodeubiquitination via intramolecular interactions to couteract monoubiquitination at the nuclear localization signal (NLS), thereby protecting it from cytoplasmic sequestration (PubMed:24703950). Acts as a tumor suppressor.

NCBI and Uniprot Product Information

• NCBI GI #: 68565074
• NCBI GeneID: 8314
• UniProt Accession #: Q92560; Q6LEM0; Q7Z5E8; A8K993
• Molecular Weight: 80,362 Da
• NCBI Official Full Name: Ubiquitin carboxyl-terminal hydrolase BAP1
• NCBI Official Synonym Full Names: BRCA1 associated protein 1
• NCBI Official Symbol: BAP1
• NCBI Official Synonym Symbols: UCHL2; hucep-6; HUCEP-13
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase BAP1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase BAP1
• Protein Family: BON1-associated protein
• UniProt Gene Name: BAP1
• UniProt Synonym Gene Names: KIAA0272
• UniProt Entry Name: BAP1_HUMAN

NCBI Description

This gene belongs to the ubiquitin C-terminal hydrolase subfamily of deubiquitinating enzymes that are involved in the removal of ubiquitin from proteins. The encoded enzyme binds to the breast cancer type 1 susceptibility protein (BRCA1) via the RING finger domain of the latter and acts as a tumor suppressor. In addition, the enzyme may be involved in regulation of transcription, regulation of cell cycle and growth, response to DNA damage and chromatin dynamics. Germline mutations in this gene may be associated with tumor predisposition syndrome (TPDS), which involves increased risk of cancers including malignant mesothelioma, uveal melanoma and cutaneous melanoma. [provided by RefSeq, May 2013]

Uniprot Description

BAP1: Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'- linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor. Component of the PR-DUB complex, at least composed of BAP1 and ASXL1. Interacts with BRCA1 (via the RING finger). Interacts (via HBM-like motif) with HCFC1. Highly expressed in testis, placenta and ovary. Expressed in breast. Belongs to the peptidase C12 family. BAP1 subfamily.

Protein type: Tumor suppressor; Ubiquitin conjugating system; Protease; EC 3.4.19.12; Ubiquitin-specific protease

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: chromatin binding; protein binding; ubiquitin-specific protease activity

Biological Process: negative regulation of cell proliferation; protein deubiquitination; regulation of cell cycle; regulation of cell growth

Disease: Tumor Predisposition Syndrome