ATP7B blocking peptide

ATP7B Antibody (C-term) Blocking Peptide

• Gene Names: ATP7B; WD; PWD; WC1; WND
• Synonyms: ATP7B; ATP7B Antibody (C-term) Blocking Peptide; Copper-transporting ATPase 2; Copper pump 2; Wilson disease-associated protein; WND/140 kDa; PWD; WC1; WND; ATP7B blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human ATP7B. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 1465

• Cellular Location: Golgi apparatus, trans-Golgi network membrane; Multi-pass membrane protein. Note: Predominantly found in the trans-Golgi network (TGN). Not redistributed to the plasma membrane in response to elevated copper levels Isoform 2: Cytoplasm
• Tissue Location: Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver The cleaved form WND/140 kDa is found in liver cell lines and other tissues
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ATP7B blocking peptide

Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile.

NCBI and Uniprot Product Information

• NCBI GI #: 239938919
• NCBI GeneID: 540
• UniProt Accession #: P35670; Q16318; Q16319; Q4U3V3; Q59FJ9; Q5T7X7
• Molecular Weight: 155,125 Da
• NCBI Official Full Name: Copper-transporting ATPase 2
• NCBI Official Synonym Full Names: ATPase copper transporting beta
• NCBI Official Symbol: ATP7B
• NCBI Official Synonym Symbols: WD; PWD; WC1; WND
• NCBI Protein Information: copper-transporting ATPase 2
• UniProt Protein Name: Copper-transporting ATPase 2
• UniProt Gene Name: ATP7B
• UniProt Synonym Gene Names: PWD; WC1; WND
• UniProt Entry Name: ATP7B_HUMAN

NCBI Description

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq, Jul 2008]

Uniprot Description

ATP7B: Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. Monomer. Interacts with COMMD1/MURR1. Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Transporter; Transporter, ion channel; Membrane protein, multi-pass; Membrane protein, integral; Hydrolase; EC 3.6.3.54

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: basolateral plasma membrane; cytoplasmic membrane-bound vesicle; Golgi membrane; integral to plasma membrane; late endosome; membrane; perinuclear region of cytoplasm; trans-Golgi network; trans-Golgi network membrane

Molecular Function: ATP binding; copper ion binding; copper-exporting ATPase activity; copper-transporting ATPase activity; protein binding

Biological Process: cellular copper ion homeostasis; copper ion import; copper ion transport; response to copper ion; sequestering of calcium ion

Disease: Wilson Disease