ASH2 blocking peptide

ASH2 Blocking Peptide

Cat. Num. AAA9626849

• Gene Names: ASH2L; ASH2; Bre2; ASH2L1; ASH2L2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: ASH2; ASH2 Blocking Peptide; ASH 2; Ash2 (absent small or homeotic) like; Ash2 (absent; small; or homeotic) like (Drosophila); ASH2 LIKE; ASH2 like protein; ASH2-like protein; Ash2l; ASH2L_HUMAN; ASH2L1; antibody; ASH2L2; Bre 2; Bre2; Discs 2-like; Drosophila absent; or homeotic; Drosophila ASH2-like; Set1/Ash2 histone methyltransferase complex subunit ASH2; ASH2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for ASH2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ASH2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 157412282
• NCBI GeneID: 9070
• NCBI Accession #: NP_001098684.1
• NCBI GenBank Nucleotide #: NM_001105214.2
• UniProt Accession #: Q9UBL3; O60659; O60660; Q96B62; A8K7C3; D3DSW9
• Molecular Weight: 60,208 Da
• NCBI Official Full Name: set1/Ash2 histone methyltransferase complex subunit ASH2 isoform b
• NCBI Official Synonym Full Names: ash2 (absent, small, or homeotic)-like (Drosophila)
• NCBI Official Symbol: ASH2L
• NCBI Official Synonym Symbols: ASH2; Bre2; ASH2L1; ASH2L2
• NCBI Protein Information: set1/Ash2 histone methyltransferase complex subunit ASH2
• UniProt Protein Name: Set1/Ash2 histone methyltransferase complex subunit ASH2
• Protein Family: Set1 complex component
• UniProt Gene Name: ASH2L
• UniProt Synonym Gene Names: ASH2L1
• UniProt Entry Name: ASH2L_HUMAN

Uniprot Description

ASH2L: Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis. Interacts with HCFC1. Core component of several methyltransferase-containing complexes including MLL1/MLL, ASCOM, MLL2/MLL3 and MLL3/MLL4. Each complex is at least composed of ASH2L, RBBP5, DPY30, WDR5, one or more specific histone methyltransferases (MLL, MLL2, MLL3 and MLL4), and the facultative components C16orf53/PA1, C17orf49, CHD8, E2F6, HCFC1, HCFC2, HSP70, INO80C, KDM6A, KANSL1, LAS1L, MAX, MCRS1, MEN1, MGA, KAT8/MOF, NCOA6, PAXIP1/PTIP, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with DPY30 and RBBP5; the interaction is direct. Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Interacts with SETD1A and SETD1B. Ubiquitously expressed. Predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; Transcription regulation

Chromosomal Location of Human Ortholog: 8p11.2

Cellular Component: nucleoplasm; histone methyltransferase complex; nucleus

Molecular Function: protein binding; DNA binding; histone lysine N-methyltransferase activity (H3-K4 specific); metal ion binding

Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; regulation of transcription, DNA-dependent; transcription, DNA-dependent; response to estrogen stimulus; positive regulation of cell proliferation; histone H3-K4 methylation; positive regulation of transcription from RNA polymerase II promoter; hemopoiesis; response to DNA damage stimulus