ARRS blocking peptide

ARRS Blocking Peptide

Cat. Num. AAA9627940

• Gene Names: SAG; RP47; S-AG
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: ARRS; ARRS Blocking Peptide; 48 kDa protein; Arrestin 1; Arrestin; ARRS_HUMAN; Retinal S antigen (48 KDa protein); Retinal S-antigen; Rod photoreceptor arrestin; RP47; S antigen; S antigen retina and pineal gland (arrestin); S arrestin; S-AG; S-arrestin; SAG; ARRS blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for ARRS blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ARRS blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 116008178
• NCBI GeneID: 6295
• NCBI Accession #: NP_000532.2
• NCBI GenBank Nucleotide #: NM_000541.4
• UniProt Accession #: P10523; Q53SV3; Q99858; A0FDN6
• Molecular Weight: 45,120 Da
• NCBI Official Full Name: S-arrestin
• NCBI Official Synonym Full Names: S-antigen; retina and pineal gland (arrestin)
• NCBI Official Symbol: SAG
• NCBI Official Synonym Symbols: RP47; S-AG
• NCBI Protein Information: S-arrestin
• UniProt Protein Name: S-arrestin
• UniProt Gene Name: SAG
• UniProt Synonym Gene Names: S-AG
• UniProt Entry Name: ARRS_HUMAN

NCBI Description

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. S-arrestin, also known as S-antigen, is a major soluble photoreceptor protein that is involved in desensitization of the photoactivated transduction cascade. It is expressed in the retina and the pineal gland and inhibits coupling of rhodopsin to transducin in vitro. Additionally, S-arrestin is highly antigenic, and is capable of inducing experimental autoimmune uveoretinitis. Mutations in this gene have been associated with Oguchi disease, a rare autosomal recessive form of night blindness. [provided by RefSeq, Jul 2008]

Uniprot Description

SAG: Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated- phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase. Retina and pineal gland. Belongs to the arrestin family.

Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: cytosol; photoreceptor inner segment; photoreceptor outer segment

Molecular Function: opsin binding; phosphoprotein binding; protein phosphatase inhibitor activity

Biological Process: cell surface receptor linked signal transduction; negative regulation of catalytic activity; phototransduction, visible light; regulation of rhodopsin mediated signaling; rhodopsin mediated signaling; visual perception

Disease: Oguchi Disease 1; Retinitis Pigmentosa 47