AP2A2 blocking peptide

AP2A2 Blocking Peptide

Cat. Num. AAA9626668

• Gene Names: AP2A2; HIP9; HYPJ; ADTAB; HIP-9; CLAPA2
• Applications: Blocking/Control
• Purity: The purity is >90%,tested by HPLC and MS.
• Synonyms: AP2A2; AP2A2 Blocking Peptide; 100 kDa coated vesicle protein C; Adaptor protein complex AP-2 subunit alpha-2; Adaptor-related protein complex 2 subunit alpha-2; Alpha-adaptin C; Alpha2-adaptin; AP-2 complex subunit alpha-2; Ap2a2; AP2A2_HUMAN; Clathrin assembly protein complex 2 alpha-C large chain; HIP-9; Huntingtin yeast partner J; Huntingtin-interacting protein 9; Huntingtin-interacting protein J; Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit; AP2A2 blocking peptide

• Purity/Purification: The purity is >90%,tested by HPLC and MS.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.

• Applicable Applications for AP2A2 blocking peptide: Blocking/Control
• Notes: For phospho antibody, we provide phospho peptide (0.5mg) and non-phospho peptide (0.5mg)
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for AP2A2 blocking peptide

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (immunoblot) and immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or immunostaining performed with the neutralized antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 338827685
• NCBI GeneID: 161
• NCBI Accession #: NP_001229766.1
• NCBI GenBank Nucleotide #: NM_001242837.1
• UniProt Accession #: O94973; O75403; Q53ET1; Q96SI8
• Molecular Weight: 73,128 Da
• NCBI Official Full Name: AP-2 complex subunit alpha-2 isoform 1
• NCBI Official Synonym Full Names: adaptor-related protein complex 2, alpha 2 subunit
• NCBI Official Symbol: AP2A2
• NCBI Official Synonym Symbols: HIP9; HYPJ; ADTAB; HIP-9; CLAPA2
• NCBI Protein Information: AP-2 complex subunit alpha-2; 100 kDa coated vesicle protein C; adapter-related protein complex 2 subunit alpha-2; adaptin, alpha B; adaptor-related protein complex 2 subunit alpha-2; alpha-adaptin C; Huntingtin interacting protein J; alpha2-adaptin; clat
• UniProt Protein Name: AP-2 complex subunit alpha-2
• Protein Family: AP-2 complex
• UniProt Gene Name: AP2A2
• UniProt Synonym Gene Names: ADTAB; CLAPA2; HIP9; HYPJ; KIAA0899; HIP-9

Uniprot Description

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif ().