ADAM17 blocking peptide

ADAM17 Antibody (N-term) Blocking Peptide

• Gene Names: ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
• Synonyms: ADAM17; ADAM17 Antibody (N-term) Blocking Peptide; Disintegrin and metalloproteinase domain-containing protein 17; ADAM 17; Snake venom-like protease; TNF-alpha convertase; TNF-alpha-converting enzyme; CD156b; CSVP; TACE; ADAM17 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human ADAM17. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 824

• Cellular Location: Membrane; Single-pass type I membrane protein
• Tissue Location: Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ADAM17 blocking peptide

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2.

NCBI and Uniprot Product Information

• NCBI GI #: 14423632
• NCBI GeneID: 6868
• UniProt Accession #: P78536; O60226
• Molecular Weight: 78,543 Da
• NCBI Official Full Name: Disintegrin and metalloproteinase domain-containing protein 17
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 17
• NCBI Official Symbol: ADAM17
• NCBI Official Synonym Symbols: CSVP; TACE; NISBD; ADAM18; CD156B; NISBD1
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 17
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 17
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM17
• UniProt Synonym Gene Names: CSVP; TACE; ADAM 17
• UniProt Entry Name: ADA17_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The encoded preproprotein is proteolytically processed to generate the mature protease. The encoded protease functions in the ectodomain shedding of tumor necrosis factor-alpha, in which soluble tumor necrosis factor-alpha is released from the membrane-bound precursor. This protease also functions in the processing of numerous other substrates, including cell adhesion proteins, cytokine and growth factor receptors and epidermal growth factor (EGF) receptor ligands. The encoded protein also plays a prominent role in the activation of the Notch signaling pathway. Elevated expression of this gene has been observed in specific cell types derived from psoriasis, rheumatoid arthritis, multiple sclerosis and Crohn's disease patients, suggesting that the encoded protein may play a role in autoimmune disease. [provided by RefSeq, Feb 2016]

Uniprot Description

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described.

Protein type: Membrane protein, integral; EC 3.4.24.86; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: actin cytoskeleton; apical plasma membrane; cell surface; cytoplasm; focal adhesion; integral to plasma membrane; intercellular junction; lipid raft; membrane; plasma membrane

Molecular Function: integrin binding; interleukin-6 receptor binding; metalloendopeptidase activity; metallopeptidase activity; Notch binding; PDZ domain binding; protein binding

Biological Process: B cell differentiation; cell adhesion; cell adhesion mediated by integrin; cell motility involved in cell locomotion; defense response to Gram-positive bacterium; epidermal growth factor receptor signaling pathway; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; germinal center formation; membrane protein ectodomain proteolysis; membrane protein intracellular domain proteolysis; negative regulation of transforming growth factor beta receptor signaling pathway; neutrophil mediated immunity; Notch receptor processing; PMA-inducible membrane protein ectodomain proteolysis; positive regulation of cell growth; positive regulation of cell migration; positive regulation of cell motility; positive regulation of cell proliferation; positive regulation of chemokine production; positive regulation of epidermal growth factor receptor activity; positive regulation of leukocyte chemotaxis; positive regulation of protein amino acid phosphorylation; positive regulation of transforming growth factor beta receptor signaling pathway; proteolysis; regulation of mast cell apoptosis; response to drug; response to high density lipoprotein stimulus; response to hypoxia; response to lipopolysaccharide; spleen development; T cell differentiation in the thymus; tumor necrosis factor-mediated signaling pathway; wound healing, spreading of epidermal cells

Disease: Inflammatory Skin And Bowel Disease, Neonatal, 1