ACTN1 blocking peptide

ACTN1 Antibody (N-term) Blocking Peptide

• Gene Names: ACTN1; BDPLT15
• Synonyms: ACTN1; ACTN1 Antibody (N-term) Blocking Peptide; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; F-actin cross-linking protein; Non-muscle alpha-actinin-1; ACTN1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human ACTN1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 892

• Cellular Location: Cytoplasm, cytoskeleton Cytoplasm, myofibril, sarcomere, Z line. Cell membrane. Cell junction. Cell projection, ruffle Note: Colocalizes with MYOZ2 and PPP3CA at the Z-line of heart and skeletal muscle. Colocalizes with PSD in membrane ruffles and central reticular structures (By similarity).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for ACTN1 blocking peptide

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.

NCBI and Uniprot Product Information

• NCBI GI #: 46397817
• NCBI GeneID: 87
• UniProt Accession #: P12814; Q1HE25; Q9BTN1; B3V8S3; B4DHH3; B7TY16
• Molecular Weight: 107,141 Da
• NCBI Official Full Name: Alpha-actinin-1
• NCBI Official Synonym Full Names: actinin alpha 1
• NCBI Official Symbol: ACTN1
• NCBI Official Synonym Symbols: BDPLT15
• NCBI Protein Information: alpha-actinin-1
• UniProt Protein Name: Alpha-actinin-1
• Protein Family: Alpha-actinin
• UniProt Gene Name: ACTN1
• UniProt Entry Name: ACTN1_HUMAN

NCBI Description

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ACTN1: a cytoskeletal protein of the spectrin superfamily. An actin-binding protein with multiple roles in different cell types. A nonmuscle isoform structurally similar to the erythroid beta spectrin gene.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q24|14q22-q24

Cellular Component: actin filament; cell projection; cytoplasm; cytosol; extracellular region; extracellular space; focal adhesion; intercellular junction; intracellular; pseudopodium; ruffle; stress fiber

Molecular Function: actin filament binding; double-stranded RNA binding; integrin binding; ligand-dependent nuclear receptor transcription coactivator activity; protein binding; protein homodimerization activity; vinculin binding

Biological Process: actin filament network formation; actin filament organization; focal adhesion formation; negative regulation of cell motility; platelet activation; platelet degranulation; platelet formation

Disease: Bleeding Disorder, Platelet-type, 15