AAK1 blocking peptide

AAK1 Antibody (C-term) Blocking Peptide

• Synonyms: AAK1; AAK1 Antibody (C-term) Blocking Peptide; AP2-associated protein kinase 1; Adaptor-associated kinase 1; KIAA1048; AAK1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human AAK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 961

• Cellular Location: Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Note: Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells (By similarity).
• Tissue Location: Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for AAK1 blocking peptide

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.

NCBI and Uniprot Product Information

• NCBI GI #: 300669613
• NCBI GeneID: 22848
• UniProt Accession #: Q2M2I8; Q4ZFZ3; Q53RX6; Q9UPV4
• Molecular Weight: 93,578 Da
• NCBI Official Full Name: AP2-associated protein kinase 1
• NCBI Official Synonym Full Names: AP2 associated kinase 1
• NCBI Official Symbol: AAK1
• NCBI Protein Information: AP2-associated protein kinase 1
• UniProt Protein Name: AP2-associated protein kinase 1
• Protein Family: AP2-associated protein kinase
• UniProt Gene Name: AAK1
• UniProt Synonym Gene Names: KIAA1048
• UniProt Entry Name: AAK1_HUMAN

NCBI Description

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

AAK1: an NAK kinase. Plays a regulatory role in clathrin-mediated endocytosis, including the regulation of fatty acid synthesis and cholesterol synthesis.

Protein type: Protein kinase, Other; EC 2.7.11.1; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Other group; NAK family

Chromosomal Location of Human Ortholog: 2p14

Cellular Component: clathrin-coated vesicle; cytoplasm; extrinsic to plasma membrane; leading edge; terminal button

Molecular Function: Notch binding; protein binding; protein serine/threonine kinase activity

Biological Process: positive regulation of Notch signaling pathway; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein stabilization; regulation of protein localization