Mouse Bloom syndrome ELISA Kit | BLM elisa kit

Mouse Bloom syndrome protein (BLM) ELISA Kit

Cat. Num. AAA284562

• Reactivity: Mouse
• Synonyms: Bloom syndrome; Mouse Bloom syndrome protein (BLM) ELISA Kit; BS; RECQ2; RECQL2; RECQL3; RecQ Helicase-Like; DNA helicase; RecQ-like type 2; BLM elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse BLM. No significant cross-reactivity or interference between Mouse BLM and analogues was observed.
• Sequence Length: 1042

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for BLM elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate BLM in samples. An antibody specific for BLM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBLM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BLM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BLM bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 110225360
• NCBI GeneID: 12144
• NCBI Accession #: NP_001035992.1
• NCBI GenBank Nucleotide #: NM_001042527.2
• UniProt Accession #: O88700; O88198
• Molecular Weight: 158,366 Da
• NCBI Official Full Name: Bloom syndrome protein homolog isoform 2
• NCBI Official Synonym Full Names: Bloom syndrome, RecQ like helicase
• NCBI Official Symbol: Blm
• NCBI Protein Information: Bloom syndrome protein homolog
• UniProt Protein Name: Bloom syndrome protein homolog
• Protein Family: Bloom syndrome protein
• UniProt Gene Name: Blm
• UniProt Synonym Gene Names: mBLM

Uniprot Description

BLM: a magnesium-dependent ATP-dependent DNA-helicase that unwinds single- and double-stranded DNA in a 3'-5' direction. A member of the RecQ helicase family that is required for genome stability. Participates in DNA replication, recombination and repair. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex which is a dynamic process changing throughout the cell cycle and within subnuclear domains. Interacts with ubiquitinated FANCD2.

Protein type: DNA repair, damage; DNA replication; EC 3.6.4.12; Helicase

Chromosomal Location of Human Ortholog: 7 D2|7 45.65 cM

Cellular Component: chromosome, telomeric region; cytoplasm; cytosol; intracellular; lateral element; male germ cell nucleus; nuclear chromosome; nuclear matrix; nucleolus; nucleus; PML body; pronucleus; replication fork

Molecular Function: ATP binding; ATP-dependent 3'-5' DNA helicase activity; ATP-dependent DNA helicase activity; ATP-dependent helicase activity; ATPase activity; bubble DNA binding; catalytic activity; DNA binding; DNA helicase activity; DNA-dependent ATPase activity; forked DNA-dependent helicase activity; four-way junction DNA binding; four-way junction helicase activity; G-quadruplex DNA binding; helicase activity; hydrolase activity; hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; metal ion binding; nucleic acid binding; nucleotide binding; p53 binding; protein binding; protein homodimerization activity; single-stranded DNA binding; telomeric D-loop binding; Y-form DNA binding; zinc ion binding

Biological Process: alpha-beta T cell differentiation; cellular metabolic process; cellular response to DNA damage stimulus; cellular response to ionizing radiation; chromosome organization and biogenesis; DNA double-strand break processing; DNA duplex unwinding; DNA recombination; DNA repair; DNA replication; G-quadruplex DNA unwinding; mitotic cell cycle G2/M transition DNA damage checkpoint; negative regulation of cell division; negative regulation of DNA recombination; negative regulation of mitotic recombination; positive regulation of alpha-beta T cell proliferation; positive regulation of double-strand break repair via homologous recombination; positive regulation of transcription, DNA-templated; protein homooligomerization; protein oligomerization; regulation of binding; regulation of cyclin-dependent protein kinase activity; regulation of DNA-dependent DNA replication; replication fork processing; replication-born double-strand break repair via sister chromatid exchange; response to X-ray; telomere maintenance; telomeric D-loop disassembly