Human Livin ELISA Kit | BIRC7 elisa kit

Human Livin PicoKine ELISA Kit

Cat. Num. AAA177226

• Gene Names: BIRC7; KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• Reactivity: Human
• Synonyms: Livin; Human Livin PicoKine ELISA Kit; Baculoviral IAP repeat-containing protein 7; baculoviral IAP repeat containing 7; Baculoviral IAP repeat containing 7; Baculoviral IAP repeat containing protein 7; BIRC 7; BIRC7; BIRC7_HUMAN; KIAP; Kidney inhibitor of apoptosis protein; LIVIN; Livin inhibitor of apotosis; Melanoma inhibitor of apoptosis protein; ML IAP; ML-IAP; MLIAP; RING finger protein 50; RNF 50; RNF50; BIRC7 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human Livin
• Sequence Length: 298

• Samples: Cell culture supernates, serum, Cell lysates and tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.78ng/ml-50ng/ml
• Sensitivity: < 20pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human Livin PicoKine ELISA Kit standard curve )

Related Product Information for BIRC7 elisa kit

Principle of the assay: human Livin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Livin has been precoated onto 96-well plates. Standards(E.coli, M1-S298) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Livin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Livin amount of sample captured in plate.
Background: BIRC7, also called KIAP or Livin, is a protein that in humans is encoded by the BIRC7 gene. This gene is mapped to 20q13.33. It encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. BIRC7 blocked apoptosis induced by BAX induction of cytochrome C release from mitochondria, and it also can block apoptosis induced by chemotherapeutic agents.

NCBI and Uniprot Product Information

• NCBI GI #: 21759008
• NCBI GeneID: 79444
• UniProt Accession #: Q96CA5; Q9BQV0; Q9H2A8; Q9HAP7
• Molecular Weight: 30,866 Da
• NCBI Official Full Name: Baculoviral IAP repeat-containing protein 7
• NCBI Official Synonym Full Names: baculoviral IAP repeat containing 7
• NCBI Official Symbol: BIRC7
• NCBI Official Synonym Symbols: KIAP; LIVIN; MLIAP; RNF50; ML-IAP
• NCBI Protein Information: baculoviral IAP repeat-containing protein 7; RING finger protein 50; livin inhibitor of apoptosis; kidney inhibitor of apoptosis protein; melanoma inhibitor of apoptosis protein
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 7
• Protein Family: Baculoviral IAP repeat-containing protein
• UniProt Gene Name: BIRC7
• UniProt Synonym Gene Names: KIAP; LIVIN; MLIAP; RNF50; KIAP; ML-IAP; Truncated livin; p30-Livin; tLivin
• UniProt Entry Name: BIRC7_HUMAN

NCBI Description

This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jul 2013]

Uniprot Description

BIRC7: Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing. Binds to CASP9. Interaction with DIABLO/SMAC via the BIR domain disrupts binding to CASP9 and apoptotic suppressor activity. Interacts with TAB1. In vitro, interacts with CASP3 and CASP7 via its BIR domain. Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes. Belongs to the IAP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligase; Ubiquitin ligase; Apoptosis; Ubiquitin conjugating system; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: Golgi apparatus; spindle microtubule; cytoplasm; nucleus

Molecular Function: protein binding; enzyme binding; zinc ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; cysteine protease inhibitor activity; ligase activity

Biological Process: protein ubiquitination; regulation of signal transduction; activation of JNK activity; regulation of cell proliferation; negative regulation of apoptosis