BirA recombinant protein

Recombinant BirA protein, His-Tag

Cat. Num. AAA388221

• Gene Names: birA; bioR; dhbB; ECK3965; JW3941
• Purity: The recombinant protein is >90% pure by SDS-PAGE.
• Synonyms: BirA; Recombinant BirA protein; His-Tag; BirA recombinant protein

• Host: E Coli
• Purity/Purification: The recombinant protein is >90% pure by SDS-PAGE.
• Form/Format: Full length recombinant BirA with His-Tag was expressed in E Coli cells and is provided in 25mM Tris pH8.0, 150mM NaCl, 5% glycerol.

• Protein Species: E Coli
• Tag: His-Tag
• Notes: Recombinant BirA is suitable for use in a variety of labeling applications and conditions. Reaction Conditions The amount of BirA required per reaction is dependent on the molecular weight of the target protein that contains the Biotinylation tag. In general, per 10nmol of target substrate to be biotinylated, 2.5ug BirA is suitable for labeling in a 40uM reaction. An example reaction is shown below for labeling 100ug of a 150 kDa antibody (0.67nmol) at 1mg/ml. In this case, although the concentration of the substrate is less than 40uM, efficient labeling can be achieved in a range of 2-8uM using as little as 0.3ug of BirA. Example reaction for labeling 150 kDa antibody with an avidin tag: 100ul antibody (1mg/ml) 20ul 0.5M Bicine pH 8.3 20ul 10X Reaction Buffer (100mM ATP, 100mM MgOAc, 500uM d-biotin) 0.5ug (1.5mg/mL BirA) 59.5ul ddH20 - Total: 200ul Incubate at 30 degrees for 2 hrs. Room temperature reactions are possible if the molarity of the substrate is sufficiently high. Increasing the concentration of BirA enzyme can also be employed to accelerate the reaction.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

Related Product Information for BirA recombinant protein

Short Description: Recombinant BirA (NP_418404.1) with His-Tag was expressed in E Coli and contains an N-terminal 6xHis-tag with an observed molecular weight of 38 kDa. The recombinant protein is >90% pure by SDS-PAGE. Recombinant BirA is suitable for use in a variety of labeling applications.

Background: BirA (bifunctional biotin-[acetylCoA carboxylase] holoenzyme synthetase/ DNA-binding transcriptional repressor, bio-5'-AMP-binding) is a bifunctional protein that exhibits biotin ligase activity and also acts as the DNA binding transcriptional repressor of the biotin operon. Biotinyl-5'-AMP is the co-repressor and is also synthesized by BirA.

Product Categories/Family for BirA recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 16131807
• NCBI GeneID: 948469
• NCBI Accession #: NP_418404.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P06709; Q2M8R4
• Molecular Weight: 35,312 Da
• NCBI Official Full Name: bifunctional biotin-[acetylCoA carboxylase] holoenzyme synthetase/ DNA-binding transcriptional repressor, bio-5'-AMP-binding
• NCBI Official Symbol: birA
• NCBI Official Synonym Symbols: bioR; dhbB; ECK3965; JW3941
• NCBI Protein Information: bifunctional biotin-[acetylCoA carboxylase] holoenzyme synthetase/ DNA-binding transcriptional repressor, bio-5'-AMP-binding
• UniProt Protein Name: Bifunctional ligase/repressor BirA
• Protein Family: Bifunctional ligase/repressor
• UniProt Gene Name: birA

NCBI Description

Biotinyl-5'-AMP is the co-repressor and is also synthesized by BirA (Cronan, 1988). [More information is available at EcoGene: EG10123]. BirA is a bifunctional protein that exhibits biotin ligase activity and also acts as the DNA binding transcriptional repressor of the biotin operon . [More information is available at EcoCyc: EG10123].

Uniprot Description

Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon.