Typical Testing Data/Standard Curve (for reference only)
Rat bone gamma-carboxyglutamate (gla) protein ELISA Kit | BGLAP elisa kit
Rat Osteocalcin/Bone gla protein, OT/BGP ELISA Kit
Reactivity
Rat
Synonyms
bone gamma-carboxyglutamate (gla) protein; Rat Osteocalcin/Bone gla protein; OT/BGP ELISA Kit; OT/BGP ELISA kit; RP11-54H19.5; BGP; OC; PMF1; bone Gla protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin) ; gamma-carboxyglutamic acid-containing protein; osteocalcin; BGLAP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat BGLAP. No significant cross-reactivity or interference between Rat BGLAP and analogues was observed.
Sequence Length
49
Samples
Serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range
0.312 pg/ml-20 pg/ml
Sensitivity
0.078 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 3
Biomineralization
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BGLAP elisa kit
Introduction: Osteocalcin is a noncollagenous protein found in bone and dentin. It is secreted by osteoblasts and thought to play a role in mineralization and calcium ion homeostasis. It has been stipulated that osteocalcin may also function as a negative regulator of bone formation, although its exact role is unknown. As osteocalcin is manufactured by osteoblasts, it is often used as a biochemical marker, or biomarker, for the bone formation process. It has been routinely observed that higher serum-osteocalcin levels are relatively well correlated with increases in bone mineral density (BMD) during treatment with anabolic bone formation drugs for osteoporosis, such as Forteo. In many studies, Osteocalcin is used as a preliminary biomarker on the effectiveness of a given drug on bone formation.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for OT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain OT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of OT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for OT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain OT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of OT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
5,431 Da
NCBI Official Full Name
Osteocalcin
UniProt Protein Name
Osteocalcin
Protein Family
UniProt Gene Name
BGLAP
UniProt Synonym Gene Names
BGP
UniProt Entry Name
OSTCN_RABIT
Uniprot Description
Function: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
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Product Notes
The Rat BGLAP bglap (Catalog #AAA705066) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA705066 ELISA Kit recognizes Rat BGLAP. It is sometimes possible for the material contained within the vial of "bone gamma-carboxyglutamate (gla) protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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