Monkey Brain Finger Protein ELISA Kit | BFP elisa kit
Monkey Brain Finger Protein ELISA Kit
Reactivity
Monkey
Synonyms
Brain Finger Protein; Monkey Brain Finger Protein ELISA Kit; BFP elisa kit
Reactivity
Monkey
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Related Product Information for BFP elisa kit
Principle of the assay: BFP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BFP antibody and an BFP-HRP conjugate. The assay sample and buffer are incubated together with BFP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BFP concentration since BFP from samples and BFP-HRP conjugate compete for the anti-BFP antibody binding site. Since the number of sites is limited, as more sites are occupied by BFP from the sample, fewer sites are left to bind BFP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BFP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
68,355 Da
NCBI Official Full Name
brain finger protein, partial
UniProt Protein Name
RING finger protein 112
UniProt Gene Name
RNF112
UniProt Synonym Gene Names
BFP; ZNF179
UniProt Entry Name
RN112_HUMAN
Uniprot Description
RNF112: 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Ubiquitin conjugating system; Hydrolase
Chromosomal Location of Human Ortholog: 17p11.2
Cellular Component: cytoplasm; integral to membrane; nucleus
Molecular Function: GTPase activity; GTP binding; zinc ion binding
Biological Process: metabolic process; positive regulation of glial cell differentiation; positive regulation of neuron differentiation
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Product Notes
The Monkey BFP rnf112 (Catalog #AAA753271) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA753271 ELISA Kit recognizes Monkey BFP. It is sometimes possible for the material contained within the vial of "Brain Finger Protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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