Typical Testing Data/Standard Curve (for reference only)
Human beta2 Glycoprotein 1antibody IgM ELISA Kit | beta2 GP1 Ab IgM elisa kit
Human beta2 Glycoprotein 1antibody IgM ELISA Kit
Reactivity
Human
Synonyms
beta2 Glycoprotein 1antibody IgM; Human beta2 Glycoprotein 1antibody IgM ELISA Kit; Human b2 Glycoprotein 1antibody IgM ELISA Kit; beta2 GP1 Ab IgM elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of B2GPIgM. No significant cross-reactivity or interference between B2GPIgM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between B2GPIgM and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for beta2 GP1 Ab IgM elisa kit
Intended Uses: This B2GPIgM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human B2GPIgM. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: B2GPIgM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-B2GPIgM antibody and an B2GPIgM-HRP conjugate. The assay sample and buffer are incubated together with B2GPIgM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the B2GPIgM concentration since B2GPIgM from samples and B2GPIgM-HRP conjugate compete for the anti-B2GPIgM antibody binding site. Since the number of sites is limited, as more sites are occupied by B2GPIgM from the sample, fewer sites are left to bind B2GPIgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The B2GPIgM concentration in each sample is interpolated from this standard curve.
Principle of the Assay: B2GPIgM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-B2GPIgM antibody and an B2GPIgM-HRP conjugate. The assay sample and buffer are incubated together with B2GPIgM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the B2GPIgM concentration since B2GPIgM from samples and B2GPIgM-HRP conjugate compete for the anti-B2GPIgM antibody binding site. Since the number of sites is limited, as more sites are occupied by B2GPIgM from the sample, fewer sites are left to bind B2GPIgM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The B2GPIgM concentration in each sample is interpolated from this standard curve.
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Product Notes
The Human beta2 GP1 Ab IgM (Catalog #AAA728040) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA728040 ELISA Kit recognizes Human beta2 GP1 Ab IgM. It is sometimes possible for the material contained within the vial of "beta2 Glycoprotein 1antibody IgM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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