Canine 1, 3-beta-D-glucan ELISA Kit | BDG elisa kit
Canine 1, 3-beta-D-glucan ELISA Kit
Reactivity
Canine
Synonyms
1; 3-beta-D-glucan; Canine 1; 3-beta-D-glucan ELISA Kit; 3-b-D-glucan ELISA Kit; BDG elisa kit
Reactivity
Canine
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Standard Curve/Testing Data
Related Product Information for BDG elisa kit
Intended Uses: This BDG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine BDG. This ELISA kit for research use only!
Principle of the Assay: BDG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BDG antibody and an BDG-HRP conjugate. The assay sample and buffer are incubated together with BDG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BDG concentration since BDG from samples and BDG-HRP conjugate compete for the anti-BDG antibody binding site. Since the number of sites is limited, as more sites are occupied by BDG from the sample, fewer sites are left to bind BDG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BDG concentration in each sample is interpolated from this standard curve.
Principle of the Assay: BDG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BDG antibody and an BDG-HRP conjugate. The assay sample and buffer are incubated together with BDG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BDG concentration since BDG from samples and BDG-HRP conjugate compete for the anti-BDG antibody binding site. Since the number of sites is limited, as more sites are occupied by BDG from the sample, fewer sites are left to bind BDG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BDG concentration in each sample is interpolated from this standard curve.
