Sheep beta Defensins ELISA Kit | BDF elisa kit

Sheep beta Defensins ELISA Kit

Cat. Num. AAA23668

• Gene Names: DEFB103B; BD-3; HBD3; HBP3; DEFB3; HBD-3; HBP-3; DEFB-3; DEFB103
• Reactivity: Sheep
• Synonyms: beta Defensins; Sheep beta Defensins ELISA Kit; BDF elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes recombinant and natural Sheep BDF, no significant cross-reactivity or interference was observed.
• Sequence Length: 67

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Detection Range: 25-800 pg/ml.
• Sensitivity: 2.0 pg/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Sheep BDF in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Sheep BDF concentration. The concentration of Sheep BDF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) are less than 15%.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BDF elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 8923890
• NCBI GeneID: 55894
• NCBI Accession #: NP_061131.1
• NCBI GenBank Nucleotide #: NM_018661.4
• UniProt Accession #: P81534; Q8NFG6; Q9NPF6
• Molecular Weight: 7,697 Da
• NCBI Official Full Name: beta-defensin 103
• NCBI Official Synonym Full Names: defensin, beta 103B
• NCBI Official Symbol: DEFB103B
• NCBI Official Synonym Symbols: BD-3; HBD3; HBP3; DEFB3; HBD-3; HBP-3; DEFB-3; DEFB103
• NCBI Protein Information: beta-defensin 103; beta-defensin 3; defensin, beta 103; defensin-like protein
• UniProt Protein Name: Beta-defensin 103
• UniProt Gene Name: DEFB103A
• UniProt Synonym Gene Names: BD3; DEFB103; DEFB3; BD-3; DEFB-3; HBD3; hBD-3
• UniProt Entry Name: D103A_HUMAN

NCBI Description

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103B, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. [provided by RefSeq, Jul 2008]

Uniprot Description

defensin, beta 3: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. Belongs to the beta-defensin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: Golgi lumen; extracellular region

Biological Process: defense response to bacterium; innate immune response; positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria