Ku70 & Ku80 Heterodimer Cell Lysate | XRCC5 cell lysate
Human Ku70 & Ku80 Heterodimer Baculovirus-Insect cells Overexpression Lysate
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Samples are stable for up to twelve months from date of receipt.
This Human Ku70 & Ku80 Heterodimer overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of Ku70 & Ku80 Heterodimer protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008]
Uniprot Description
Ku80: the 80-kilodalton subunit of the Ku complex, also known as ATP-dependant DNA helicase II. A single stranded DNA-dependent ATP-dependent helicase. It functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.
Protein type: RNA-binding; DNA-binding; EC 3.6.4.-; Nuclear receptor co-regulator; Helicase; Nucleolus; EC 3.6.1.-
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: nucleoplasm; nuclear telomere cap complex; membrane; nuclear chromosome, telomeric region; nucleolus; plasma membrane; nucleus; cytosol
Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; telomeric DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding
Biological Process: response to drug; cell proliferation; positive regulation of neurogenesis; transcription, DNA-dependent; viral reproduction; double-strand break repair; positive regulation of interferon type I production; innate immune response; brain development; DNA repair; negative regulation of transcription, DNA-dependent; telomere maintenance; double-strand break repair via nonhomologous end joining; DNA duplex unwinding; DNA recombination
Research Articles on XRCC5
Similar Products
Product Notes
The XRCC5 xrcc5 (Catalog #AAA8118072) is a Cell Lysate produced from Baculovirus-Insect cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1379 (751 + 628)aa. AAA Biotech's Ku70 & Ku80 Heterodimer can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the XRCC5 xrcc5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ile73 2, Met1-Asp60 9. It is sometimes possible for the material contained within the vial of "Ku70 & Ku80 Heterodimer, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
