Glypican 5 Active Protein | GPC5 active protein

Glypican 5 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8119989

• Purity: >92% as determined by SDS-PAGE
• Synonyms: Glypican 5; Glypican 5 Protein; Human; Recombinant (His Tag); Human Glypican 5/GPC5 Protein (His Tag); glypican 5; GPC5 active protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >92% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 100mM NaCl, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr554

• Species: Human
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human GPC5 at 5ug/ml (100uL/well) can bind human bFGF with a linear range of 0.156-2.5ng/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human GPC5 (NP_004457.1)(Met 1-Thr 554) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized human GPC5 at 5 ug/ml (100 ul/well) can bind human bFGF with a linear ranger of 0.156-2.5 ng/ml.)

Related Product Information for GPC5 active protein

Background: Glypican-5 (GPC5), is a cell membrane protein that belongs to the glypican family. The glypicans compose a family of glycosylphosphatidylinositol-anchored heparan sulfate proteoglycans that may play a role in the control of cell division and growth regulation. So far, six members (Glypican-1/GPC1, Glypican-2/GPC2, Glypican-3/GPC3, Glypican-4/GPC4, Glypican-5/GPC5, Glypican-6/GPC6) of this family are known in the vertebrates. In the adult, Glypican-5 is primarily expressed in the brain. It is also detected in the fetal brain, lung, and liver. Glypican-5 enhances the intracellular signaling of FGF2 and HGF. It alters the cellular distribution of FGF2. The properties of Glypican-5 make it an attractive target for therapeutic intervention in rhabdomyosarcomas and other tumors that amplify and/or overexpress its gene. Glypican-5 is overexpressed in lymphoma cell lines that had shown amplification. It is a likely target for amplification, and that over-expression of GPC5 may contribute to the development and/or progression of lymphomas and other tumors.

References

Yu W, et al. (2003) GPC5 is a possible target for the 13q31-q32 amplification detected in lymphoma cell lines. J Hum Genet. 48(6): 331-5.Williamson D, et al. (2007) Role for amplification and expression of glypican-5 in rhabdomyosarcoma. Cancer Res. 67(1): 57-65.

NCBI and Uniprot Product Information

• NCBI GI #: 4758464
• NCBI GeneID: 2262
• NCBI Accession #: NP_004457.1
• NCBI GenBank Nucleotide #: NM_004466.4
• UniProt Accession #: P78333; O60436; Q9BX27; B2R726
• Molecular Weight: 63,707 Da
• NCBI Official Full Name: glypican-5
• NCBI Official Synonym Full Names: glypican 5
• NCBI Official Symbol: GPC5
• NCBI Protein Information: glypican-5; bA93M14.1; glypican proteoglycan 5
• UniProt Protein Name: Glypican-5
• Protein Family: Glypican
• UniProt Gene Name: GPC5
• UniProt Entry Name: GPC5_HUMAN

NCBI Description

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. [provided by RefSeq, Jul 2008]

Uniprot Description

GPC5: Cell surface proteoglycan that bears heparan sulfate. Belongs to the glypican family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Cell surface

Chromosomal Location of Human Ortholog: 13q32

Cellular Component: lysosomal lumen; extracellular space; proteinaceous extracellular matrix; integral to plasma membrane; Golgi lumen; plasma membrane; extracellular region

Molecular Function: heparan sulfate proteoglycan binding

Biological Process: chondroitin sulfate metabolic process; phototransduction, visible light; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; retinoid metabolic process