CD39 Cell Lysate | ENTPD1 cell lysate
Human CD39 Baculovirus-Insect Overexpression Lysate
Gene Names
ENTPD1; CD39; SPG64; ATPDase; NTPDase-1
Applications
Western Blot
Synonyms
CD39; Human CD39 Baculovirus-Insect Overexpression Lysate; Human CD39/ENTPD1 (ECD) Insect Cell Lysate (WB positive control); ectonucleoside triphosphate diphosphohydrolase 1; Human ATPDase Overexpression Lysate; Human CD39 Overexpression Lysate; Human NTPDase-1 Overexpression Lysate; Human SPG64 Overexpression Lysate; ENTPD1 cell lysate
Host
Baculovirus-Insect Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
452aa
Sequence
Thr38-Val478
Applicable Applications for ENTPD1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human ENTPD1 (NP_001767.3) (Thr38-Val478) was expressed with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for ENTPD1 cell lysate
CD39, also known as ENTPD1, belongs to the GDA1/CD39 NTPase family. It is expressed primarily on activated lymphoid cells and can also be detected in endothelial tissues. The vascular isoform and the placental isoform II are present in both placenta and umbilical vein, whereas placental isoform I is present in placenta only. CD39 can hydrolyze both nucleoside triphosphates and diphosphates. It is the dominant ecto nucleotidase of vascular and placental trophoblastic tissues and appears to modulate the functional expression of type 2 purinergic (P2) G protein coupled receptors (GPCRs). CD39 transgenic mice exhibit impaired platelet aggregation, prolonged bleeding times, and resistance to systemic thromboembolism. There is a correlation between ATP hydrolysis and triglycerides in patients with chronic heart disease, suggesting a relationship between ATP diphosphohydrolase and thrombogenesis. In the nervous system, CD39 could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission.
This Human CD39 overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of CD39 protein from the overexpression lysate was verified.
This Human CD39 overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of CD39 protein from the overexpression lysate was verified.
Product Categories/Family for ENTPD1 cell lysate
References
Kunzli BM, et al. (2011) Variable impact of CD39 in experimental murine colitis. Dig Dis Sci. 2011 56 (5): 1393-403.Clayton A, et al. (2011) Cancer exosomes express CD39 and CD73, which suppress T cells through adenosine production. J Immunol. 187 (2): 676-83.Loza MJ, et al. (2011) T-cell specific defect in expression of the NTPDase CD39 as a biomarker for lupus. Cell Immunol. 271 (1): 110-7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
59,325 Da
NCBI Official Full Name
ectonucleoside triphosphate diphosphohydrolase 1 isoform 1
NCBI Official Synonym Full Names
ectonucleoside triphosphate diphosphohydrolase 1
NCBI Official Symbol
ENTPD1
NCBI Official Synonym Symbols
CD39; SPG64; ATPDase; NTPDase-1
NCBI Protein Information
ectonucleoside triphosphate diphosphohydrolase 1
UniProt Protein Name
Ectonucleoside triphosphate diphosphohydrolase 1
Protein Family
UniProt Gene Name
ENTPD1
UniProt Synonym Gene Names
CD39; NTPDase 1; Ecto-ATPDase 1
UniProt Entry Name
ENTP1_HUMAN
NCBI Description
The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
Uniprot Description
ENTPD1: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. Belongs to the GDA1/CD39 NTPase family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Nucleotide Metabolism - purine; EC 3.6.1.5; Extracellular matrix; Nucleotide Metabolism - pyrimidine; Membrane protein, multi-pass; Hydrolase
Chromosomal Location of Human Ortholog: 10q24
Cellular Component: membrane; integral to plasma membrane; plasma membrane; basal lamina
Molecular Function: protein binding; nucleoside-diphosphatase activity; nucleoside-triphosphatase activity; ATP binding
Biological Process: platelet activation; G-protein coupled receptor protein signaling pathway; purine ribonucleoside diphosphate catabolic process; cell adhesion; blood coagulation
Disease: Spastic Paraplegia 64, Autosomal Recessive
Research Articles on ENTPD1
Similar Products
Product Notes
The ENTPD1 entpd1 (Catalog #AAA8115605) is a Cell Lysate produced from Baculovirus-Insect Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 452aa. AAA Biotech's CD39 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the ENTPD1 entpd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Thr38-Val4 78. It is sometimes possible for the material contained within the vial of "CD39, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
